100-401-N97S

antibody from Rockland Immunochemicals, Inc.

Targeting: MORC3 KIAA0136, NXP2, ZCW5, ZCWCC3

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

100-401-N97S

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#100-401-N97S, RRID:AB_2610794

Product name

Anti-Morc3 (RABBIT) Antibody - 100-401-N97S

Antibody type

Polyclonal

Vial size

25 µl

References

Submitted references

MORC3, a novel MIWI2 association partner, as an epigenetic regulator of piRNA dependent transposon silencing in male germ cells.

Kojima-Kita K, Kuramochi-Miyagawa S, Nakayama M, Miyata H, Jacobsen SE, Ikawa M, Koseki H, Nakano T
Scientific reports 2021 Oct 14;11(1):20472

---

Mouse MORC3 is a GHKL ATPase that localizes to H3K4me3 marked chromatin.

Li S, Yen L, Pastor WA, Johnston JB, Du J, Shew CJ, Liu W, Ho J, Stender B, Clark AT, Burlingame AL, Daxinger L, Patel DJ, Jacobsen SE
Proceedings of the National Academy of Sciences of the United States of America 2016 Aug 30;113(35):E5108-16

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western Blot of Rabbit anti-Morc3 antibody. Lane 1: Human embryonic stem cell. Lane 2: Human embryonic stem cell. Lane 3: C-Flag Mouse embryonic stem cell. Lane 4: C-Flag Mouse embryonic stem cell doxycycline induced. Load: 35 µg per lane. Primary antibody: hMorc3 antibody at 1:1000-1:5000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 107kDa/ ~170kDa. Other band(s): sumoylated Morc run higher.

Validation comment

Western Blot