Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00004952-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00004952-M02, RRID:AB_608931
- Product name
- Mouse Monoclonal OCRL Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. OCRL - oculocerebrorenal syndrome of Lowe
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: OCRL Antibody (4A6) [H00004952-M02] - Analysis of OCRL over-expressed 293 cell line, cotransfected with OCRL Validated Chimera RNAi ( Cat # H00004952-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with OCRL monoclonal antibody (M02), clone 4A6 (Cat # H00004952-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: OCRL Antibody (4A6) [H00004952-M02] - Analysis of OCRL expression in transfected 293T cell line by OCRL monoclonal antibody (M02), clone 4A6.Lane 1: OCRL transfected lysate(103.2 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: OCRL Antibody (4A6) [H00004952-M02] - OCRL monoclonal antibody (M02), clone 4A6 Analysis of OCRL expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: OCRL Antibody (4A6) [H00004952-M02] - Detection limit for recombinant GST tagged OCRL is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: OCRL Antibody (4A6) [H00004952-M02] - Analysis of monoclonal antibody to OCRL on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.