PA5-44889

antibody from Invitrogen Antibodies

Targeting: ACTN1

Antibody data

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Validation data

Product number: PA5-44889 - Provider product page
Provider: Invitrogen Antibodies
Product name: alpha Actinin 1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Peptide sequence: DHYDSQQTND YMQPEEDWDR DLLLDPAWEK QQRKTFTAWC NSHLRKAGTQ Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 75%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 85%
Reactivity: Human, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.5 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

ACTN1 protein structure - PA5-44889 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of rACTNX-GFP cell lysate using an anti-alpha Actinin polyclonal antibody (Product # PA5-44889). Primary Antibody Dilution: 1:1000; Secondary Antibody: Anti-rabbit HRP; Secondary Antibody Dilution: 1:5000. LANE 1) 10 µg ACTN1-GFP transfected COS-7 lysate; LANE 2) 10 µg ACTN2-GFP transfected COS-7 lysate; LANE 3) 10 µg ACTN3-GFP transfected COS-7 lysate; LANE 4) 10 µg ACTN4-GFP transfected COS-7 lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of SW480 (Lane 1), Caco-2 (Lane 2), A-431 (Lane 3), HeLa (Lane 4), A549 (Lane 5), MCF7 (Lane 6), COS-7 (Lane 7), NIH/3T3 (Lane 8) and HEK-293 (Lane 9). The blot was probed with Anti-alpha Actinin Polyclonal Antibody (Product # PA5-44889, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 110 kDa band corresponding to alpha Actinin was detected across the cell lines tested except for HEK-293 which is reported negative for alpha Actinin expression.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of rat cortical neurons using an anti-alpha Actinin polyclonal antibody (Product # PA5-44889). Primary antibody dilution: 1:1000-1:2000; Secondary antibody: HRP conjugated goat anti-rabbit; Secondary antibody dilution: 1:20,000. LANE1: Rat cortical neurons ctr siRNA (30µg); LANE2: Rat cortical neurons ACTN1 siRNA (30µg).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of human HEK293 cells using an anti-alpha Actinin polyclonal antibody (Product # PA5-44889).; Secondary Antibody: HRP conjugated goat anti-rabbit.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of human 293T cell lysate using an anti-alpha Actinin polyclonal antibody (Product # PA5-44889).