Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-85791 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-85791, RRID:AB_11022015
- Product name
- Rabbit Polyclonal alpha-Actinin 1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of alpha-Actinin 1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E
Nature methods 2013 Apr;10(4):315-23
Nature methods 2013 Apr;10(4):315-23
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha-Actinin 1 Antibody [NBP1-85791] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha-Actinin 1 Antibody [NBP1-85791] - Analysis in human cell lines A-431 and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha-Actinin 1 Antibody [NBP1-85791] - Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody [NBP1-85791] - Staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.