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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- PA5-27870 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- alpha Actinin 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: PC-12, Rat2, rat brain, 293T, A431, HeLa, HepG2, NIH-3T3. Predicted reactivity: Mouse (100%), Rat (98%), Zebrafish (98%), Xenopus laevis (97%), Pig (98%), Chicken (97%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 0.09 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using Alpha-actinin 1 (Product # PA5-27870) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
