PA5-27870

antibody from Invitrogen Antibodies

Targeting: ACTN1

Antibody data

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Validation data

Product number: PA5-27870 - Provider product page
Provider: Invitrogen Antibodies
Product name: alpha Actinin 1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: PC-12, Rat2, rat brain, 293T, A431, HeLa, HepG2, NIH-3T3. Predicted reactivity: Mouse (100%), Rat (98%), Zebrafish (98%), Xenopus laevis (97%), Pig (98%), Chicken (97%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.09 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

ACTN1 protein structure - PA5-27870 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using alpha Actinin 1 Polyclonal Antibody (Product # PA5-27870). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. 7.5% SDS PAGE. Alpha Actinin 1 Polyclonal Antibody (Product # PA5-27870) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of alpha Actinin 1 was performed by separating 50 µg of Rat tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a alpha Actinin 1 Polyclonal Antibody (Product # PA5-27870) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of alpha Actinin 1 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a alpha Actinin 1 Polyclonal Antibody (Product # PA5-27870) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using alpha Actinin 1 Polyclonal Antibody (Product # PA5-27870). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. 7.5% SDS PAGE. Alpha Actinin 1 Polyclonal Antibody (Product # PA5-27870) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: alpha Actinin 1 antibody detects alpha Actinin 1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 1 antibody alpha Actinin 1 Polyclonal Antibody (Product # PA5-27870) diluted at a dilution of 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.