BS-1056R

antibody from Invitrogen Antibodies

Targeting: HTR2C 5-HT2C, 5HTR2C, HTR1C

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

BS-1056R

Provider

Invitrogen Antibodies

Product name

5-HTR2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

L1 rat brain, L2 human colon carcinoma lysates probed (bs-1056R) at 1:200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90 min in 37°C. Predicted band and observed band size: 52 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti 5-HTR2A/HTR2A Polyclonal Antibody, Unconjugated (bs-1056R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with 5-HT2A Polyclonal Antibody, Unconjugated (bs-1056R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.