PA5-87944

antibody from Invitrogen Antibodies

Targeting: RASGRP1 CalDAG-GEFII, RASGRP

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-87944

Provider

Invitrogen Antibodies

Product name

RASGRP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: PVAPTENNTS VGPVSNLCSL GAKDLLHAPE EGPFTFPNGE AVEHGEESKD RTIMLMGVSS QKISLRLKRA VAHKATQTES QPWIGSEGPS GPFVLSSPRK TAQDTLYVLP SPTSPCPSPV LVRKRAFVKW ENKDSLIKSK EELRHLRLPT YQELEQEINT LKADNDALKI QLKYAQKKIE SLQLEKSNHV LAQMEQGDCS; Positive Samples: Jurkat, MCF7, A-549, U-251MG, SW480; Cellular Location: Cell membrane, Cytoplasm, Endoplasmic reticulum membrane, Golgi apparatus membrane, Peripheral membrane protein, cytosol

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.48 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RASGRP1 in extracts of THP-1 cells. Samples were incubated with RASGRP1 Polyclonal antibody (Product # PA5-87944) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of RASGRP1 in A-549 cells. Samples were incubated with RASGRP1 Polyclonal antibody (Product # PA5-87944) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of RASGRP1 in C6 cells. Samples were incubated with RASGRP1 Polyclonal antibody (Product # PA5-87944) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of RASGRP1 in NIH/3T3 cells. Samples were incubated with RASGRP1 Polyclonal antibody (Product # PA5-87944) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.