13690-1-AP

antibody from Proteintech Group

Targeting: NEDD4L KIAA0439, NEDD4-2, RSP5

Provider product page for 13690-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [64]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [2]

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Product number: 13690-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#13690-1-AP, RRID:AB_2149326
Product name: NEDD4L antibody
Antibody type: Polyclonal
Description: KD/KO validated NEDD4L antibody (Cat. #13690-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

NEDD4L protein structure - 13690-1-AP shown in red.

Submitted references Development and external validation of a mitophagy-related diagnostic model for biliary atresia based on cellular infiltration patterns.

Sun D, Zhang J, Zhao Y, Li S, Zhang Y, Liao J, Wang D, Hua K, Gu Y, Du J, Zhang S, Huang J
Translational pediatrics 2026 Feb 28;15(2):27

DPSC-EVs Drive Functional Recovery in Sjögren's Disease by Systemic Immunomodulation.

Kawado T, Ogata K, Moriyama M, Yoshioka H, Yano A, Matsumura M, Nakamura S, Kawano S
Tissue engineering. Part A 2026 Feb 25;:19373341261426389

Silencing NEDD4L Effectively Inhibits the Malignant Behaviors of Hepatocellular Carcinoma.

Dai J, Zhu T, Wan J, Liu R, Song Y, Zhang D, Wang X, Zhang H, Li W, Lin J, Zhu X, Liu Y
Journal of hepatocellular carcinoma 2025;12:1369-1391

eIF3f promotes tumour malignancy by remodelling fatty acid biosynthesis in hepatocellular carcinoma.

Zhou S, Zhang L, You Y, Yu K, Tie X, Gao Y, Chen Y, Yao F, Zhang R, Hao X, Fang C, Li X, Li Q, Wang X
Journal of hepatology 2025 Sep;83(3):712-728

Activating NEDD4L suppresses EGFR-driven lung adenocarcinoma growth via facilitating EGFR proteasomal degradation.

Chen M, Jiang W, Zhan J, Zhang S, Zheng J, Huang Y, He J, Yang Y, Zhao S, Zhang Y, Liu J, Pang L, Zhang L, Fang W, Li J
Journal of experimental & clinical cancer research : CR 2025 Oct 21;44(1):294

Loss of CFIm activates YAP/TAZ and connects mRNA cleavage and polyadenylation inhibition to BRCAness.

Goldthorpe M, Hou J, Nadendla A, Osmanbeyoglu HU, Hwang HW
bioRxiv : the preprint server for biology 2025 Oct 21;

Munc13-4 mediates tumor immune evasion by regulating the sorting and secretion of PD-L1 via exosomes.

Liu C, Liu D, Zheng X, Guan J, Zhou X, Zhang H, Wang S, Li Q, Lu G, He J, Ma C
Nature communications 2025 Oct 13;16(1):9080

SPINT2 inhibits NEDD4L-mediated ACSL4 ubiquitination to promote ferroptosis and suppress gallbladder cancer progression.

Ye P, Yang X, Wang H, Ren Y, Gao M, Wang Y, Guo Y, Sun Y, Xu J
International journal of biological macromolecules 2025 Nov;330(Pt 3):148141

Machine learning-powered discovery of a novel berberine derivative inducing SCD-dependent ferroptosis in osteosarcoma.

He M, Liu Y, Li T, Liu Y, Wang X, Xie J, Wang A, Wang Y, Yuan Y, Cui M, Du Z
Journal of translational medicine 2025 Nov 20;23(1):1328

ELK1 inhibition alleviates amyloid pathology and memory decline by promoting the SYVN1-mediated ubiquitination and degradation of PS1 in Alzheimer's disease.

Yi L, Li J, He Y, Wang J, Wang M, Guo S, Luo M, Wu B, Xu M, Tian Q, Fan Y, Chen M, Xu B, Xia L, Song W, He G, Du Y, Dong Z
Experimental & molecular medicine 2025 May;57(5):1032-1046

Endothelial major vault protein alleviates vascular remodeling via promoting Parkin-mediated mitophagy.

Jiang B, Bai F, Hu Y, Ren Y, Su Y, Song W, Xie K, Wang D, Pan J, Liu Y, Feng Y, Li X, Zhang H, Zhu X, Bai H, Yang Q, Ben J, Chen Q
Nature communications 2025 May 10;16(1):4365

Elevated Astrocytic NFAT5 of the hippocampus increases epilepsy susceptibility in hypoxic-ischemic mice.

Jia X, Xu J, Zhang Y, Kong S, Cheng X, Wu N, Han S, Yin J, Liu W, He X, Fan Y, Liu Y, Chen T, Peng B
Epilepsia 2025 Mar;66(3):817-832

A New target of ischemic ventricular arrhythmias-ITFG2.

Pan QM, Bi FF, Jing ZH, Cao M, Cui C, Liu F, Jin L, Yi-Jie H, Tian H, Yu T, Yun W, Shan HL, Zhou YH
European journal of pharmacology 2025 Mar 15;991:177301

Exosomal miR-196a-5p Secreted by Bone Marrow Mesenchymal Stem Cells Inhibits Ferroptosis and Promotes Drug Resistance of Acute Myeloid Leukemia.

Fan B, Wang L, Hu T, Zheng L, Wang J
Antioxidants & redox signaling 2025 Jun;42(16-18):933-953

Arginine methyltransferase PRMT1 promotes ferroptosis through EGR1/GLS2 axis in sepsis-related acute lung injury.

Li M, Hu L, Ke Q, Li Z, Ruan C, Lu H, Liu X
Communications biology 2025 Feb 3;8(1):159

NEDD4L-Mediated Ubiquitination of GPX4 Exacerbates Doxorubicin-Induced Cardiotoxicity.

Ke J, Li L, Chen S, Liao C, Peng F, Chai D, Lin J
International journal of molecular sciences 2025 Aug 23;26(17)

Resveratrol induces ferroptosis in triple-negative breast cancer through NEDD4L-mediated GPX4 ubiquitination and degradation.

Zhang E, Wang Y, Zhang H, Li X, Su Y, Cui J, Xu R, Mao X, Sang M, Lin Z, Zhou X
Free radical biology & medicine 2025 Aug 1;235:231-247

µMap proximity labeling in living cells reveals stress granule disassembly mechanisms.

Pan CR, Knutson SD, Huth SW, MacMillan DWC
Nature chemical biology 2025 Apr;21(4):490-500

Ubiquitin-specific protease 1 facilitates hepatocellular carcinoma progression by modulating mitochondrial fission and metabolic reprogramming via cyclin-dependent kinase 5 stabilization.

Bian S, Ni W, Zhou L, Tong Y, Dai C, Zhao X, Qiang Y, Gao J, Xiao Y, Liu W, Chen C, Lin S, Gong J, Zhao S, Chen Y, Lin Z, Liu D, Zhao H, Zheng W
Cell death and differentiation 2024 Sep;31(9):1202-1218

NPR1 promotes cisplatin resistance by inhibiting PARL-mediated mitophagy-dependent ferroptosis in gastric cancer.

Wu C, Wang S, Huang T, Xi X, Xu L, Wang J, Hou Y, Xia Y, Xu L, Wang L, Huang X
Cell biology and toxicology 2024 Oct 30;40(1):93

NEDD4L Inhibits the Proliferation and Migration of Keloid Fibroblasts by Regulating YY1 Ubiquitination-Mediated Glycolytic Metabolic Reprogramming.

Jin J, Wang K, Lu C, Yao C, Xie F
Experimental dermatology 2024 Nov;33(11):e70008

SGK1 contributes to ferroptosis in coronary heart disease through the NEDD4L/NF-κB pathway.

Peng Y, Jiang Y, Zhou Q, Jia Z, Tang H
Journal of molecular and cellular cardiology 2024 Nov;196:71-83

Candesartan upregulates angiotensin-converting enzyme 2 in kidneys of male animals by decreased ubiquitination.

Wang P, Ren Z, Wang W, Liu M, Jia Y, Zhang M, Xue Y, Zhang C, Xu J, Wang C, Wang X
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2024 Mar 31;38(6):e23537

NEDD4L mediates ITGB4 ubiquitination and degradation to suppress esophageal carcinoma progression.

Shi Y, Fang N, Wu Y, Xu H, Ning A, Zhang W, Liu Y, Tao X, Chen Q, Tian T, Zhang L, Chu M, Cui J
Cell communication and signaling : CCS 2024 Jun 3;22(1):302

USP25 Elevates SHLD2-Mediated DNA Double-Strand Break Repair and Regulates Chemoresponse in Cancer.

Li Y, Li L, Wang X, Zhao F, Yang Y, Zhou Y, Zhang J, Wang L, Jiang Z, Zhang Y, Chen Y, Wu C, Li K, Zhang T, Wang P, Mao Z, Zhu W, Xu X, Liang S, Lou Z, Yuan J
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Jul;11(28):e2403485

The E3 ubiquitin ligase Nedd4L preserves skeletal muscle stem cell quiescence by inhibiting their activation.

Blackburn DM, Sahinyan K, Hernández-Corchado A, Lazure F, Richard V, Raco L, Perron G, Zahedi RP, Borchers CH, Lepper C, Kawabe H, Jahani-Asl A, Najafabadi HS, Soleimani VD
iScience 2024 Jul 19;27(7):110241

The Upregulation of Leucine-Rich Repeat Containing 1 Expression Activates Hepatic Stellate Cells and Promotes Liver Fibrosis by Stabilizing Phosphorylated Smad2/3.

Wang Y, Li X, Guan X, Song Z, Liu H, Guan Z, Wang J, Zhu L, Zhang D, Zhao L, Xie P, Wei X, Shang N, Liu Y, Jin Z, Ji Z, Dai G
International journal of molecular sciences 2024 Feb 27;25(5)

Maternal fiber-rich diet promotes early-life intestinal development in offspring through milk-derived extracellular vesicles carrying miR-146a-5p.

Lu D, Liu Y, Kang L, Zhang X, Hu J, Ye H, Huang B, Wu Y, Zhao J, Dai Z, Wang J, Han D
Journal of nanobiotechnology 2024 Feb 16;22(1):65

DOPAMINE D4 RECEPTOR DOWN-REGULATES RENAL SODIUM CHLORIDE COTRANSPORTER VIA UBIQUITINATION-ASSOCIATED LYSOSOME DEGRADATION.

Zhang M, Liu M, Ren Z, Wang W, Upadhyay K, Asico LA, Armando I, Jia Y, Wang P, Xue Y, Wang X
bioRxiv : the preprint server for biology 2024 Feb 16;

ITFG2 as a NEDD4-2 inhibitor: Preserving calcium homeostasis to prevent myocardial ischemic injury.

Liu F, Lv LF, Bi FF, Pan QM, Jing ZH, Cui C, Cao M, Yu T, Li J, He YJ, Xiao HW, Tian H, Wu Y, Shan HL, Zhou YH
Biochemical pharmacology 2024 Dec;230(Pt 2):116597

ITFG2, an immune-modulatory protein, targets ATP 5b to maintain mitochondrial function in myocardial infarction.

Bi FF, Cao M, Pan QM, Jing ZH, Lv LF, Liu F, Tian H, Yu T, Li TY, Li XL, Liang HH, Shan HL, Zhou YH
Biochemical pharmacology 2024 Aug;226:116338

A Novel Ubiquitin Ligase Adaptor PTPRN Suppresses Seizure Susceptibility through Endocytosis of Na(V)1.2 Sodium Channels.

Wang Y, Yang H, Li N, Wang L, Guo C, Ma W, Liu S, Peng C, Chen J, Song H, Chen H, Ma X, Yi J, Lian J, Kong W, Dong J, Tu X, Shah M, Tian X, Huang Z
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Aug;11(29):e2400560

E3 ubiquitin ligase NEDD4L inhibits epithelial-mesenchymal transition by suppressing the β-catenin/HIF-1α positive feedback loop in chronic rhinosinusitis with nasal polyps.

Chen SY, Liu PQ, Qin DX, Lv H, Zhou HQ, Xu Y
Acta pharmacologica Sinica 2024 Apr;45(4):831-843

HDLBP Promotes Hepatocellular Carcinoma Proliferation and Sorafenib Resistance by Suppressing Trim71-dependent RAF1 Degradation.

Yuan J, Lv T, Yang J, Wu Z, Yan L, Yang J, Shi Y, Jiang L
Cellular and molecular gastroenterology and hepatology 2023;15(2):307-325

A novel NEDD4L-TXNIP-CHOP axis in the pathogenesis of nonalcoholic steatohepatitis.

Guo Q, Xin M, Lu Q, Feng D, Yang V, Peng LF, Whelan KA, Hu W, Wu S, Yang X, Wang H, Rothberg BS, Gamero AM, Gerhard GS, Gao B, Yang L
Theranostics 2023;13(7):2210-2225

Cytoplasmic YAP1-mediated ESCRT-III assembly promotes autophagic cell death and is ubiquitinated by NEDD4L in breast cancer.

Guo Y, Cui Y, Li Y, Jin X, Wang D, Lei M, Chen F, Liu Y, Xu J, Yao G, Zeng G, Chen X
Cancer communications (London, England) 2023 May;43(5):582-612

Genetic analysis of periventricular nodular heterotopia 7 caused by a novel NEDD4L missense mutation: Case and literature summary.

Liu J, Hu J, Duan Y, Qin R, Guo C, Zhou H, Liu H, Liu C
Molecular genetics & genomic medicine 2023 Jul;11(7):e2169

A novel variant in the FBP1 gene causes fructose-1,6-bisphosphatase deficiency through increased ubiquitination.

Liang X, Liu X, Li W, Zhang L, Zhang B, Lai G, Zhao Y
Archives of biochemistry and biophysics 2023 Jul 1;742:109619

The miR-143/145 cluster induced by TGF-β1 suppresses Wilms' tumor 1 expression in cultured human podocytes.

Tabei A, Sakairi T, Hamatani H, Ohishi Y, Watanabe M, Nakasatomi M, Ikeuchi H, Kaneko Y, Kopp JB, Hiromura K
American journal of physiology. Renal physiology 2023 Jul 1;325(1):F121-F133

ARID2 mitigates hepatic steatosis via promoting the ubiquitination of JAK2.

Cao HJ, Jiang H, Ding K, Qiu XS, Ma N, Zhang FK, Wang YK, Zheng QW, Xia J, Ni QZ, Xu S, Zhu B, Ding XF, Chen TW, Qiu L, Chen W, Li ZG, Zhou B, Feng WM, Xie D, Li JJ
Cell death and differentiation 2023 Feb;30(2):383-396

Renal CD81 interacts with sodium potassium 2 chloride cotransporter and sodium chloride cotransporter in rats with lipopolysaccharide-induced preeclampsia.

Wang P, Zhu G, Wu Q, Shen L, Liu D, Wang Z, Wang W, Ren Z, Jia Y, Liu M, Xue Y, Ji D, Hu Y, Yu Y, Wang X
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2023 Apr;37(4):e22834

Paeoniflorin Regulates NEDD4L/STAT3 Pathway to Induce Ferroptosis in Human Glioma Cells.

Nie XH, Qiu S, Xing Y, Xu J, Lu B, Zhao SF, Li YT, Su ZZ
Journal of oncology 2022;2022:6093216

Nedd4-2 Haploinsufficiency in Mice Impairs the Ubiquitination of Rer1 and Increases the Susceptibility to Endoplasmic Reticulum Stress and Seizures.

Liu X, Zhang L, Zhang H, Liang X, Zhang B, Tu J, Zhao Y
Frontiers in molecular neuroscience 2022;15:919718

Pharmacological suppression of Nedd4-2 rescues the reduction of Kv11.1 channels in pathological cardiac hypertrophy.

Zhang H, Fu T, Sun J, Zou S, Qiu S, Zhang J, Su S, Shi C, Li DP, Xu Y
Frontiers in pharmacology 2022;13:942769

Targeted Degradation of 53BP1 Using Ubiquitin Variant Induced Proximity.

Aminu B, Fux J, Mallette E, Petersen N, Zhang W
Biomolecules 2022 Mar 22;12(4)

Mesenchymal Stem Cell-Derived Exosomes Attenuate Epithelial-Mesenchymal Transition of HK-2 Cells.

Yin S, Zhou S, Ren D, Zhang J, Xin H, He X, Gao H, Hou J, Zeng F, Lu Y, Zhang X, Fan M
Tissue engineering. Part A 2022 Jul;28(13-14):651-659

LINC00941 promotes pancreatic cancer malignancy by interacting with ANXA2 and suppressing NEDD4L-mediated degradation of ANXA2.

Wang J, He Z, Liu X, Xu J, Jiang X, Quan G, Jiang J
Cell death & disease 2022 Aug 18;13(8):718

TH5487, a small molecule inhibitor of OGG1, attenuates pulmonary fibrosis by NEDD4L-mediated OGG1 degradation.

Ling H, Song C, Fang Y, Yin Y, Wu Z, Wang Y, Xu Z, Gao S, Li A, Liu G
Chemico-biological interactions 2022 Aug 1;362:109999

PTENα is responsible for protection of brain against oxidative stress during aging.

Wang P, Li R, Yuan Y, Zhu M, Liu Y, Jin Y, Yin Y
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021 Oct;35(10):e21943

Piezo2 channel in nodose ganglia neurons is essential in controlling hypertension in a pathway regulated directly by Nedd4-2.

Huo L, Gao Y, Zhang D, Wang S, Han Y, Men H, Yang Z, Qin X, Wang R, Kong D, Bai H, Zhang H, Zhang W, Jia Z
Pharmacological research 2021 Feb;164:105391

Aagab acts as a novel regulator of NEDD4-1-mediated Pten nuclear translocation to promote neurological recovery following hypoxic-ischemic brain damage.

Dai C, Wu B, Chen Y, Li X, Bai Y, Du Y, Pang Y, Wang YT, Dong Z
Cell death and differentiation 2021 Aug;28(8):2367-2384

Exosomes Derived From Macrophages Enhance Aerobic Glycolysis and Chemoresistance in Lung Cancer by Stabilizing c-Myc via the Inhibition of NEDD4L.

Wang H, Wang L, Pan H, Wang Y, Shi M, Yu H, Wang C, Pan X, Chen Z
Frontiers in cell and developmental biology 2020;8:620603

JP1 suppresses proliferation and metastasis of melanoma through MEK1/2 mediated NEDD4L-SP1-Integrin αvβ3 signaling.

Cui J, Shu C, Xu J, Chen D, Li J, Ding K, Chen M, Li A, He J, Shu Y, Yang L, Zhang R, Zhou J
Theranostics 2020;10(18):8036-8050

The correlation between NEDD4L and HIF-1α levels as a gastric cancer prognostic marker.

Jiang X, Zhang S, Yin Z, Sheng Y, Yan Q, Sun R, Lu M, Zhang Z, Li Y
International journal of medical sciences 2019;16(11):1517-1524

USP38 critically promotes asthmatic pathogenesis by stabilizing JunB protein.

Chen S, Yun F, Yao Y, Cao M, Zhang Y, Wang J, Song X, Qian Y
The Journal of experimental medicine 2018 Nov 5;215(11):2850-2867

Ehrlichia chaffeensis TRP120 Moonlights as a HECT E3 Ligase Involved in Self- and Host Ubiquitination To Influence Protein Interactions and Stability for Intracellular Survival.

Zhu B, Das S, Mitra S, Farris TR, McBride JW
Infection and immunity 2017 Sep;85(9)

20-Hydroxyeicosatetraenoic acid regulates the expression of Nedd4‑2 in kidney and liver through a neddylation modification pathway.

Zhao J, Zhang B, Lai G, Xu R, Chu G, Zhao Y
Molecular medicine reports 2017 Dec;16(6):9671-9677

Mutations in the HECT domain of NEDD4L lead to AKT-mTOR pathway deregulation and cause periventricular nodular heterotopia.

Broix L, Jagline H, Ivanova E, Schmucker S, Drouot N, Clayton-Smith J, Pagnamenta AT, Metcalfe KA, Isidor B, Louvier UW, Poduri A, Taylor JC, Tilly P, Poirier K, Saillour Y, Lebrun N, Stemmelen T, Rudolf G, Muraca G, Saintpierre B, Elmorjani A, Deciphering Developmental Disorders study, Moïse M, Weirauch NB, Guerrini R, Boland A, Olaso R, Masson C, Tripathy R, Keays D, Beldjord C, Nguyen L, Godin J, Kini U, Nischké P, Deleuze JF, Bahi-Buisson N, Sumara I, Hinckelmann MV, Chelly J
Nature genetics 2016 Nov;48(11):1349-1358

Plk1 phosphorylation of PTEN causes a tumor-promoting metabolic state.

Li Z, Li J, Bi P, Lu Y, Burcham G, Elzey BD, Ratliff T, Konieczny SF, Ahmad N, Kuang S, Liu X
Molecular and cellular biology 2014 Oct 1;34(19):3642-61

Pathobiological properties of the ubiquitin ligase Nedd4L in melanoma.

Kito Y, Bai J, Goto N, Okubo H, Adachi Y, Nagayama T, Takeuchi T
International journal of experimental pathology 2014 Feb;95(1):24-8

Oncogenic function of p34SEI-1 via NEDD4‑1‑mediated PTEN ubiquitination/degradation and activation of the PI3K/AKT pathway.

Jung S, Li C, Jeong D, Lee S, Ohk J, Park M, Han S, Duan J, Kim C, Yang Y, Kim KI, Lim JS, Kang YS, Lee MS
International journal of oncology 2013 Nov;43(5):1587-95

YAP1 recruits c-Abl to protect angiomotin-like 1 from Nedd4-mediated degradation.

Skouloudaki K, Walz G
PloS one 2012;7(4):e35735

Decreased expression of Nedd4L correlates with poor prognosis in gastric cancer patient.

Gao C, Pang L, Ren C, Ma T
Medical oncology (Northwood, London, England) 2012 Sep;29(3):1733-8

Nedd4L modulates the transcription of metalloproteinase-1 and -13 genes to increase the invasive activity of gallbladder cancer.

Takeuchi T, Adachi Y, Nagayama T, Furihata M
International journal of experimental pathology 2011 Apr;92(2):79-86

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: K-562 cells were subjected to SDS PAGE followed by western blot with 13690-1-AP(NEDD4L antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human prostate cancer using 13690-1-AP(NEDD4L antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The NEDD4L antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human NEDD4L. This antibody recognizes human,mouse,rat antigen. The NEDD4L antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 13690-1-AP.pdf

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