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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- RQ4197 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- NEDD4-2 Antibody / NEDD4L
- Antibody type
- Polyclonal
- Description
- This highly specific NEDD4-2 antibody is suitable for use in Western blot/Flow cytometry/ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the NEDD4-2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human 22RV1, 2) human Jurkat, 3) rat pancreas, 4) mouse pancreas and 5) mouse NIH3T3 lysate with NEDD4-2 antibody at 0.5ug/ml. Predicted molecular weight: 112-125 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U-2 OS cells with NEDD4-2 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NEDD4-2 antibody.
