Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00023327-M04 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00023327-M04, RRID:AB_2149323
- Product name
- Mouse Monoclonal NEDD4L Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. NEDD4L (1D2)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NEDD4L Antibody (1D2) [H00023327-M04] - Analysis of NEDD4L expression in transfected 293T cell line by NEDD4L monoclonal antibody (M04), clone 1D2. Lane 1: NEDD4L transfected lysate (Predicted MW: 104.9 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: NEDD4L Antibody (1D2) [H00023327-M04] - Detection limit for recombinant GST tagged NEDD4L is 0.3 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: NEDD4L Antibody (1D2) [H00023327-M04] - Analysis of NEDD4L transfected lysate using anti-NEDD4L monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NEDD4L monoclonal antibody.