Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-21911 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MC1R Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: Molt-4. Predicted reactivity: Chimpanzee (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.96 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MC1-R using 30 µg of MOLT4 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a MC1-R polyclonal antibody (Product # PA5-21911) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MC1R Polyclonal Antibody (Product # PA5-21911). Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with MC1R Polyclonal Antibody (Product # PA5-21911) diluted at 1:4,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MC1R Polyclonal Antibody (Product # PA5-21911). Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with MC1R Polyclonal Antibody (Product # PA5-21911) diluted at 1:4,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MC1R Polyclonal Antibody detects MC1R protein at cytosol and membrane on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. MC1R Polyclonal Antibody (Product # PA5-21911) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MC1R Polyclonal Antibody detects MC1R protein at membrane on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. MC1R Polyclonal Antibody (Product # PA5-21911) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.