AMR-020-200UL

antibody from Invitrogen Antibodies

Targeting: MC1R MSH-R

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AMR-020-200UL

Provider

Invitrogen Antibodies

Product name

MC1 Receptor Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

200 µL

Concentration

0.6 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human normal skin fibroblast cell line Malme-3 (lanes 1 and 4) and human malignant melanoma cell lines Malme-3M (lanes 2 and 5) and A875 (lanes 3 and 6): - 1,2,3. Anti-MC1 Receptor Antibody (#AMR-020), (1:500).4,5,6. Anti-MC1 Receptor Antibody , preincubated with MC1 Receptor Blocking Peptide (#BLP-MR020).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human normal skin fibroblast cell line Malme-3 (lanes 1 and 4) and human malignant melanoma cell lines Malme-3M (lanes 2 and 5) and A875 (lanes 3 and 6): - 1,2,3. Anti-MC1 Receptor Antibody (#AMR-020), (1:500).4,5,6. Anti-MC1 Receptor Antibody , preincubated with MC1 Receptor Blocking Peptide (#BLP-MR020).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat adrenal lysate: - 1. Anti-MC1 Receptor Antibody (#AMR-020), (1:400). 2. Anti-MC1 Receptor Antibody , preincubated with MC1 Receptor Blocking Peptide (#BLP-MR020).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of MC1R in human Malme-3M cells - Immunocytochemical staining of human paraformaldehyde fixed and permeabilized malignant melanoma cell lines (Malme-3M). A. Cells were stained with Anti-MC1 Receptor Antibody (#AMR-020), (1:200) followed by goat- Anti-rabbit-AlexaFluor-555 secondary Antibody . B. Live view of the same field as in (A).C. Computer merge of (A) and (B).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expressionof MC1R in normal skin and melanoma - Immunohistochemical staining of paraffin embeddednormal skin and melanoma sections using Anti-MC1 Receptor Antibody (#AMR-020) (1:100).MCR1 staining (red-brown color is highly specificin A. epidermal cells, B. eccrine sweat gland cells and C. melanoma cells. Color reaction was obtained with DAB. Hematoxilin is used as the counterstain.