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LearnPA5-29932
antibody from Invitrogen Antibodies
Targeting: ATP6AP2
APT6M8-9, ATP6IP2, ATP6M8-9, M8-9, PRR, RENR
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [3]
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- Product number
- PA5-29932 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATP6IP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (92%), Rat (91%), Rhesus Monkey (98%), Bovine (87%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.38 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Renin Receptor using 30 µg of HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Renin Receptor polyclonal antibody (Product # PA5-29932) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ATP6IP2 Polyclonal Antibody (Product # PA5-29932). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP6IP2 Polyclonal Antibody (Product # PA5-29932) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of ATP6IP2 was achieved by transfecting MCF-7 with ATP6IP2 specific siRNAs (Silencer® select Product # s19791, 19790). Western blot analysis (Fig. a) was performed using whole cell extracts from the ATP6IP2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Arp3 Polyclonal Antibody (Product # PA5-29932, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to ATP6IP2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-ATP6IP2 Polyclonal Antibody (Product # PA5-29932) and a ~37 kD band was observed across the panel tested. Whole cell extracts (30 µg lysate) of MCF-7 (Lane 1), T-47D (Lane 2), PC-3 (Lane 3), SiHa (Lane 4), Panc-1 (Lane 5) and HCT 116 (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Renin Receptor in methanol-fixed HepG2 cells using a Renin Receptor polyclonal antibody (Product # PA5-29932) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Renin Receptor antibody detects Renin Receptor protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Renin Receptor stained by Renin Receptor antibody (Product # PA5-29932) diluted at 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ATP6IP2 Polyclonal Antibody detects Renin Receptor protein by immunofluorescent analysis. Sample: DIV9 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Renin Receptor stained by ATP6IP2 Polyclonal Antibody (Product # PA5-29932) diluted at 1:500. Red: Tau, a Axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .
