Antibody data
- Antibody Data
- Antigen structure
- References [42]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 15848-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#15848-1-AP, RRID:AB_10597100
- Product name
- INS antibody
- Antibody type
- Polyclonal
- Description
- INS antibody (Cat. #15848-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Neu-P11 - a novel melatonin receptor agonist, could improve the features of type-2 diabetes mellitus in rats.
JunD Regulates Pancreatic β-Cells Function by Altering Lipid Accumulation.
Meteorin-Like Ameliorates β Cell Function by Inhibiting β Cell Apoptosis of and Promoting β Cell Proliferation via Activating the WNT/β-Catenin Pathway.
SNAPIN Regulates Cell Cycle Progression to Promote Pancreatic β Cell Growth.
Glucokinase is required for high-starch diet-induced β-cell mass expansion in mice.
HGF can reduce accumulation of inflammation and regulate glucose homeostasis in T2D mice.
Type IIB PKA is highly expressed in β cells and controls cell proliferation via regulating Cyclin D1 expression.
Mof acetyltransferase inhibition ameliorates glucose intolerance and islet dysfunction of type 2 diabetes via targeting pancreatic α-cells.
Morphological and biochemical changes in the pancreas associated with acute systemic hypoxia.
12-Lipoxygenase governs the innate immune pathogenesis of islet inflammation and autoimmune diabetes.
Nfe2l1 deficiency mitigates streptozotocin-induced pancreatic β-cell destruction and development of diabetes in male mice.
Advanced glycation end products activated endothelial-to-mesenchymal transition in pancreatic islet endothelial cells and triggered islet fibrosis in diabetic mice.
Circular RNA circPPM1F modulates M1 macrophage activation and pancreatic islet inflammation in type 1 diabetes mellitus.
Depletion of the diabetic gut microbiota resistance enhances stem cells therapy in type 1 diabetes mellitus.
The MEK Inhibitor Trametinib Suppresses Major Histocompatibility Antigen-mismatched Rejection Following Pancreatic Islet Transplantation.
Tectorigenin enhances PDX1 expression and protects pancreatic β-cells by activating ERK and reducing ER stress.
Islet transplantation in the subcutaneous space achieves long-term euglycaemia in preclinical models of type 1 diabetes.
Long-Term Expansion of Pancreatic Islet Organoids from Resident Procr+ Progenitors.
Localized Controlled Release of Bilirubin from β-Cyclodextrin-Conjugated ε-Polylysine To Attenuate Oxidative Stress and Inflammation in Transplanted Islets.
FABP4 inhibitor attenuates inflammation and endoplasmic reticulum stress of islet in leptin receptor knockout rats.
Mint3 is dispensable for pancreatic and kidney functions in mice.
Using Recombinant Human Collagen With Basic Fibroblast Growth Factor to Provide a Simulated Extracellular Matrix Microenvironment for the Revascularization and Attachment of Islets to the Transplantation Region.
TLR4 gene in the regulation of periodontitis and its molecular mechanism.
Rotenone protects against β-cell apoptosis and attenuates type 1 diabetes mellitus.
Upregulation of vitamin D-binding protein is associated with changes in insulin production in pancreatic beta-cells exposed to p,p'-DDT and p,p'-DDE.
Salsalate Prevents β-Cell Dedifferentiation in OLETF Rats with Type 2 Diabetes through Notch1 Pathway.
Expression of P2X1 receptors in somatostatin-containing cells in mouse gastrointestinal tract and pancreatic islets of both mouse and human.
AWRK6, a Novel GLP-1 Receptor Agonist, Attenuates Diabetes by Stimulating Insulin Secretion.
The Synergy of Two Factors on Insulin Expression.
Hepatitis B X-interacting protein promotes the formation of the insulin gene-transcribing protein complex Pdx-1/Neurod1 in animal pancreatic β-cells.
Differential protein analysis of serum exosomes post-intravenous immunoglobulin therapy in patients with Kawasaki disease.
Involvement of Insulin Signaling Disturbances in Bisphenol A-Induced Alzheimer's Disease-like Neurotoxicity.
Secretory expression and surface display of a new and biologically active single-chain insulin (SCI-59) analog by lactic acid bacteria.
The effect of cholesteryl ester transfer protein on pancreatic beta cell dysfunction in mice.
Insulin signaling disruption in male mice due to perinatal bisphenol A exposure: Role of insulin signaling in the brain.
Bisphenol A disrupts glucose transport and neurophysiological role of IR/IRS/AKT/GSK3β axis in the brain of male mice.
Silencing Mist1 Gene Expression Is Essential for Recovery from Acute Pancreatitis.
Effects of Bisphenol A on glucose homeostasis and brain insulin signaling pathways in male mice.
CNC-bZIP protein Nrf1-dependent regulation of glucose-stimulated insulin secretion.
Autoimmune diabetes is suppressed by treatment with recombinant human tissue Kallikrein-1.
Display of human proinsulin on the Bacillus subtilis spore surface for oral administration.
Tendons from non-diabetic humans and rats harbor a population of insulin-producing, pancreatic beta cell-like cells.
Li X, He J, Li X, Li Y, Zhou Y, Cai S
Endokrynologia Polska 2021;72(6):634-642
Endokrynologia Polska 2021;72(6):634-642
JunD Regulates Pancreatic β-Cells Function by Altering Lipid Accumulation.
Wang K, Cui Y, Lin P, Yao Z, Sun Y
Frontiers in endocrinology 2021;12:689845
Frontiers in endocrinology 2021;12:689845
Meteorin-Like Ameliorates β Cell Function by Inhibiting β Cell Apoptosis of and Promoting β Cell Proliferation via Activating the WNT/β-Catenin Pathway.
Hu W, Wang R, Sun B
Frontiers in pharmacology 2021;12:627147
Frontiers in pharmacology 2021;12:627147
SNAPIN Regulates Cell Cycle Progression to Promote Pancreatic β Cell Growth.
Jiang M, Kuang Z, He Y, Cao Y, Yu T, Cheng J, Liu W, Wang W
Frontiers in endocrinology 2021;12:624309
Frontiers in endocrinology 2021;12:624309
Glucokinase is required for high-starch diet-induced β-cell mass expansion in mice.
Tsuchida K, Nakamura A, Miyoshi H, Yang K, Yamauchi Y, Kawata S, Omori K, Takahashi K, Kitao N, Nomoto H, Kameda H, Cho KY, Seino Y, Terauchi Y, Atsumi T
Journal of diabetes investigation 2021 Sep;12(9):1545-1554
Journal of diabetes investigation 2021 Sep;12(9):1545-1554
HGF can reduce accumulation of inflammation and regulate glucose homeostasis in T2D mice.
Yan L, He X, Tang Y, Zhao X, Luo G, Wang X
Journal of physiology and biochemistry 2021 Nov;77(4):613-624
Journal of physiology and biochemistry 2021 Nov;77(4):613-624
Type IIB PKA is highly expressed in β cells and controls cell proliferation via regulating Cyclin D1 expression.
Jiang Y, Zhu L, Wu D, Ni Y, Huang C, Ye H, Yang Y, Liu R, Li Y
The FEBS journal 2021 Nov 28;
The FEBS journal 2021 Nov 28;
Mof acetyltransferase inhibition ameliorates glucose intolerance and islet dysfunction of type 2 diabetes via targeting pancreatic α-cells.
Guo X, Cui C, Song J, He Q, Zang N, Hu H, Wang X, Li D, Wang C, Hou X, Li X, Liang K, Yan F, Chen L
Molecular and cellular endocrinology 2021 Nov 1;537:111425
Molecular and cellular endocrinology 2021 Nov 1;537:111425
Morphological and biochemical changes in the pancreas associated with acute systemic hypoxia.
Morioka F, Tani N, Ikeda T, Hirokawa T, Ikeda K, Shida A, Aoki Y, Ishikawa T
Human cell 2021 Mar;34(2):400-418
Human cell 2021 Mar;34(2):400-418
12-Lipoxygenase governs the innate immune pathogenesis of islet inflammation and autoimmune diabetes.
Kulkarni A, Pineros AR, Walsh MA, Casimiro I, Ibrahim S, Hernandez-Perez M, Orr KS, Glenn L, Nadler JL, Morris MA, Tersey SA, Mirmira RG, Anderson RM
JCI insight 2021 Jul 22;6(14)
JCI insight 2021 Jul 22;6(14)
Nfe2l1 deficiency mitigates streptozotocin-induced pancreatic β-cell destruction and development of diabetes in male mice.
Bao S, Zheng H, Chen C, Zhang Y, Bao L, Yang B, Hou Y, Chen Y, Zhang Q, Pi J, Fu J
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2021 Dec;158:112633
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2021 Dec;158:112633
Advanced glycation end products activated endothelial-to-mesenchymal transition in pancreatic islet endothelial cells and triggered islet fibrosis in diabetic mice.
Tsai PS, Chiu CY, Sheu ML, Yang CY, Lan KC, Liu SH
Chemico-biological interactions 2021 Aug 25;345:109562
Chemico-biological interactions 2021 Aug 25;345:109562
Circular RNA circPPM1F modulates M1 macrophage activation and pancreatic islet inflammation in type 1 diabetes mellitus.
Zhang C, Han X, Yang L, Fu J, Sun C, Huang S, Xiao W, Gao Y, Liang Q, Wang X, Luo F, Lu W, Zhou Y
Theranostics 2020;10(24):10908-10924
Theranostics 2020;10(24):10908-10924
Depletion of the diabetic gut microbiota resistance enhances stem cells therapy in type 1 diabetes mellitus.
Lv W, Graves DT, He L, Shi Y, Deng X, Zhao Y, Dong X, Ren Y, Liu X, Xiao E, Zhang Y
Theranostics 2020;10(14):6500-6516
Theranostics 2020;10(14):6500-6516
The MEK Inhibitor Trametinib Suppresses Major Histocompatibility Antigen-mismatched Rejection Following Pancreatic Islet Transplantation.
Tada S, Anazawa T, Shindo T, Yamane K, Inoguchi K, Fujimoto N, Nagai K, Masui T, Okajima H, Takaori K, Sumi S, Uemoto S
Transplantation direct 2020 Sep;6(9):e591
Transplantation direct 2020 Sep;6(9):e591
Tectorigenin enhances PDX1 expression and protects pancreatic β-cells by activating ERK and reducing ER stress.
Yao X, Li K, Liang C, Zhou Z, Wang J, Wang S, Liu L, Yu CL, Song ZB, Bao YL, Zheng LH, Sun Y, Wang G, Huang Y, Yi J, Sun L, Li Y
The Journal of biological chemistry 2020 Sep 11;295(37):12975-12992
The Journal of biological chemistry 2020 Sep 11;295(37):12975-12992
Islet transplantation in the subcutaneous space achieves long-term euglycaemia in preclinical models of type 1 diabetes.
Yu M, Agarwal D, Korutla L, May CL, Wang W, Griffith NN, Hering BJ, Kaestner KH, Velazquez OC, Markmann JF, Vallabhajosyula P, Liu C, Naji A
Nature metabolism 2020 Oct;2(10):1013-1020
Nature metabolism 2020 Oct;2(10):1013-1020
Long-Term Expansion of Pancreatic Islet Organoids from Resident Procr+ Progenitors.
Wang D, Wang J, Bai L, Pan H, Feng H, Clevers H, Zeng YA
Cell 2020 Mar 19;180(6):1198-1211.e19
Cell 2020 Mar 19;180(6):1198-1211.e19
Localized Controlled Release of Bilirubin from β-Cyclodextrin-Conjugated ε-Polylysine To Attenuate Oxidative Stress and Inflammation in Transplanted Islets.
Yao Q, Huang ZW, Zhai YY, Yue M, Luo LZ, Xue PP, Han YH, Xu HL, Kou L, Zhao YZ
ACS applied materials & interfaces 2020 Feb 5;12(5):5462-5475
ACS applied materials & interfaces 2020 Feb 5;12(5):5462-5475
FABP4 inhibitor attenuates inflammation and endoplasmic reticulum stress of islet in leptin receptor knockout rats.
Yao F, Jiang DD, Guo WH, Guo LS, Gao MM, Bai Y, Wang X, Zhang LS
European review for medical and pharmacological sciences 2020 Dec;24(24):12808-12820
European review for medical and pharmacological sciences 2020 Dec;24(24):12808-12820
Mint3 is dispensable for pancreatic and kidney functions in mice.
Chung Y, Saitoh Y, Hayashi T, Fukui Y, Terada N, Seiki M, Murakami Y, Sakamoto T
Biochemistry and biophysics reports 2020 Dec;24:100872
Biochemistry and biophysics reports 2020 Dec;24:100872
Using Recombinant Human Collagen With Basic Fibroblast Growth Factor to Provide a Simulated Extracellular Matrix Microenvironment for the Revascularization and Attachment of Islets to the Transplantation Region.
Zhu Q, Lu C, Jiang X, Yao Q, Jiang X, Huang Z, Jiang Y, Peng L, Fu H, Zhao Y
Frontiers in pharmacology 2019;10:1536
Frontiers in pharmacology 2019;10:1536
TLR4 gene in the regulation of periodontitis and its molecular mechanism.
Qi W, Yang X, Ye N, Li S, Han Q, Huang J, Wu B
Experimental and therapeutic medicine 2019 Sep;18(3):1961-1966
Experimental and therapeutic medicine 2019 Sep;18(3):1961-1966
Rotenone protects against β-cell apoptosis and attenuates type 1 diabetes mellitus.
Wu M, Chen W, Zhang S, Huang S, Zhang A, Zhang Y, Jia Z
Apoptosis : an international journal on programmed cell death 2019 Dec;24(11-12):879-891
Apoptosis : an international journal on programmed cell death 2019 Dec;24(11-12):879-891
Upregulation of vitamin D-binding protein is associated with changes in insulin production in pancreatic beta-cells exposed to p,p'-DDT and p,p'-DDE.
Pavlíková N, Daniel P, Šrámek J, Jelínek M, Šrámková V, Němcová V, Balušíková K, Halada P, Kovář J
Scientific reports 2019 Dec 2;9(1):18026
Scientific reports 2019 Dec 2;9(1):18026
Salsalate Prevents β-Cell Dedifferentiation in OLETF Rats with Type 2 Diabetes through Notch1 Pathway.
Han F, Li X, Yang J, Liu H, Zhang Y, Yang X, Yang S, Chang B, Chen L, Chang B
Aging and disease 2019 Aug;10(4):719-730
Aging and disease 2019 Aug;10(4):719-730
Expression of P2X1 receptors in somatostatin-containing cells in mouse gastrointestinal tract and pancreatic islets of both mouse and human.
Ji R, Zhu J, Wang D, Sui QQ, Knight GE, Burnstock G, Yuan H, Xiang Z
Purinergic signalling 2018 Sep;14(3):285-298
Purinergic signalling 2018 Sep;14(3):285-298
AWRK6, a Novel GLP-1 Receptor Agonist, Attenuates Diabetes by Stimulating Insulin Secretion.
Wang Q, Zhao C, Jin L, Zhang H, Miao Q, Liu H, Zhang D
International journal of molecular sciences 2018 Oct 7;19(10)
International journal of molecular sciences 2018 Oct 7;19(10)
The Synergy of Two Factors on Insulin Expression.
Luo Y, Xu Y, Wang ZY, Li X, Xing WB, Zhang TC
Cellular reprogramming 2018 Feb;20(1):49-54
Cellular reprogramming 2018 Feb;20(1):49-54
Hepatitis B X-interacting protein promotes the formation of the insulin gene-transcribing protein complex Pdx-1/Neurod1 in animal pancreatic β-cells.
Li H, Wang Z, Li Y, Fang R, Wang H, Shi H, Zhang X, Zhang W, Ye L
The Journal of biological chemistry 2018 Feb 9;293(6):2053-2065
The Journal of biological chemistry 2018 Feb 9;293(6):2053-2065
Differential protein analysis of serum exosomes post-intravenous immunoglobulin therapy in patients with Kawasaki disease.
Zhang L, Song QF, Jin JJ, Huang P, Wang ZP, Xie XF, Gu XQ, Gao XJ, Jia HL
Cardiology in the young 2017 Nov;27(9):1786-1796
Cardiology in the young 2017 Nov;27(9):1786-1796
Involvement of Insulin Signaling Disturbances in Bisphenol A-Induced Alzheimer's Disease-like Neurotoxicity.
Wang T, Xie C, Yu P, Fang F, Zhu J, Cheng J, Gu A, Wang J, Xiao H
Scientific reports 2017 Aug 8;7(1):7497
Scientific reports 2017 Aug 8;7(1):7497
Secretory expression and surface display of a new and biologically active single-chain insulin (SCI-59) analog by lactic acid bacteria.
Mao R, Wu D, Hu S, Zhou K, Wang M, Wang Y
Applied microbiology and biotechnology 2017 Apr;101(8):3259-3271
Applied microbiology and biotechnology 2017 Apr;101(8):3259-3271
The effect of cholesteryl ester transfer protein on pancreatic beta cell dysfunction in mice.
Guo W, Gong Y, Fu Z, Fu J, Sun Y, Ju X, Chang Y, Wang W, Zhu X, Gao B, Liu X, Yang T, Zhou H
Nutrition & metabolism 2016;13:21
Nutrition & metabolism 2016;13:21
Insulin signaling disruption in male mice due to perinatal bisphenol A exposure: Role of insulin signaling in the brain.
Fang F, Gao Y, Wang T, Chen D, Liu J, Qian W, Cheng J, Gao R, Wang J, Xiao H
Toxicology letters 2016 Mar 14;245:59-67
Toxicology letters 2016 Mar 14;245:59-67
Bisphenol A disrupts glucose transport and neurophysiological role of IR/IRS/AKT/GSK3β axis in the brain of male mice.
Li J, Wang Y, Fang F, Chen D, Gao Y, Liu J, Gao R, Wang J, Xiao H
Environmental toxicology and pharmacology 2016 Apr;43:7-12
Environmental toxicology and pharmacology 2016 Apr;43:7-12
Silencing Mist1 Gene Expression Is Essential for Recovery from Acute Pancreatitis.
Karki A, Humphrey SE, Steele RE, Hess DA, Taparowsky EJ, Konieczny SF
PloS one 2015;10(12):e0145724
PloS one 2015;10(12):e0145724
Effects of Bisphenol A on glucose homeostasis and brain insulin signaling pathways in male mice.
Fang F, Chen D, Yu P, Qian W, Zhou J, Liu J, Gao R, Wang J, Xiao H
General and comparative endocrinology 2015 Feb 1;212:44-50
General and comparative endocrinology 2015 Feb 1;212:44-50
CNC-bZIP protein Nrf1-dependent regulation of glucose-stimulated insulin secretion.
Zheng H, Fu J, Xue P, Zhao R, Dong J, Liu D, Yamamoto M, Tong Q, Teng W, Qu W, Zhang Q, Andersen ME, Pi J
Antioxidants & redox signaling 2015 Apr 1;22(10):819-31
Antioxidants & redox signaling 2015 Apr 1;22(10):819-31
Autoimmune diabetes is suppressed by treatment with recombinant human tissue Kallikrein-1.
Maneva-Radicheva L, Amatya C, Parker C, Ellefson J, Radichev I, Raghavan A, Charles ML, Williams MS, Robbins MS, Savinov AY
PloS one 2014;9(9):e107213
PloS one 2014;9(9):e107213
Display of human proinsulin on the Bacillus subtilis spore surface for oral administration.
Feng F, Hu P, Chen L, Tang Q, Lian C, Yao Q, Chen K
Current microbiology 2013 Jul;67(1):1-8
Current microbiology 2013 Jul;67(1):1-8
Tendons from non-diabetic humans and rats harbor a population of insulin-producing, pancreatic beta cell-like cells.
Lehner C, Gehwolf R, Wagner A, Resch H, Hirzinger C, Augat P, Stephan D, Aigner L, Rivera FJ, Bauer HC, Tempfer H
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme 2012 Jun;44(7):506-10
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme 2012 Jun;44(7):506-10
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15848-1-AP(INS antibody) at dilution of 1:10000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human pancreas using 15848-1-AP(INS antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The Insulin antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human Insulin. This antibody recognizes human,mouse,rat antigen. The Insulin antibody has been validated for the following applications: ELISA, WB, IHC analysis.