15848-1-AP

antibody from Proteintech Group

Targeting: INS IDDM1, IDDM2

Provider product page for 15848-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [68]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [2]

Submit

Product number: 15848-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#15848-1-AP, RRID:AB_10597100
Product name: INS antibody
Antibody type: Polyclonal
Description: INS antibody (Cat. #15848-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

INS protein structure - 15848-1-AP shown in red.

Submitted references Inhibition of the Eukaryotic Initiation Factor-2-α Kinase PERK Decreases Risk of Autoimmune Diabetes in Mice.

Muralidharan C, Huang F, Enriquez JR, Wang JE, Nelson JB, Nargis T, May SC, Chakraborty A, Figatner KT, Navitskaya S, Anderson CM, Calvo V, Surguladze D, Mulvihill MJ, Yi X, Sarkar S, Oakes SA, Webb-Robertson BM, Sims EK, Staschke KA, Eizirik DL, Nakayasu ES, Stokes ME, Tersey SA, Mirmira RG
bioRxiv : the preprint server for biology 2024 Jun 3;

CK2 activity is crucial for proper glucagon expression.

Ampofo E, Pack M, Wrublewsky S, Boewe AS, Spigelman AF, Koch H, MacDonald PE, Laschke MW, Montenarh M, Götz C
Diabetologia 2024 Jul;67(7):1368-1385

Maternal obstructive sleep apnea aggravates metabolic dysfunction-associated fatty liver disease via HMGB1-TLR4 signaling-mediated endoplasmic reticulum stress in male offspring rats.

Wang R, Feng W, Wang Y, Jiang Y, Lin Y, Chen X
Biochimica et biophysica acta. Molecular basis of disease 2024 Jan;1870(1):166889

SORCS2 activity in pancreatic α-cells safeguards insulin granule formation and release from glucose-stressed β-cells.

Kalnytska O, Qvist P, Kunz S, Conrad T, Willnow TE, Schmidt V
iScience 2024 Jan 19;27(1):108725

Spatiotemporal role of SETD2-H3K36me3 in murine pancreatic organogenesis.

Lu P, Xu J, Shen X, Sun J, Liu M, Niu N, Wang Q, Xue J
Cell reports 2024 Feb 27;43(2):113703

Liver ChREBP deficiency inhibits fructose-induced insulin resistance in pregnant mice and female offspring.

Li J, Zhang S, Sun Y, Li J, Feng Z, Li H, Zhang M, Yan T, Han J, Duan Y
EMBO reports 2024 Apr;25(4):2097-2117

Generation of rat forebrain tissues in mice.

Huang J, He B, Yang X, Long X, Wei Y, Li L, Tang M, Gao Y, Fang Y, Ying W, Wang Z, Li C, Zhou Y, Li S, Shi L, Choi S, Zhou H, Guo F, Yang H, Wu J
Cell 2024 Apr 25;187(9):2129-2142.e17

Inhibition of polyamine biosynthesis preserves β cell function in type 1 diabetes.

Sims EK, Kulkarni A, Hull A, Woerner SE, Cabrera S, Mastrandrea LD, Hammoud B, Sarkar S, Nakayasu ES, Mastracci TL, Perkins SM, Ouyang F, Webb-Robertson BJ, Enriquez JR, Tersey SA, Evans-Molina C, Long SA, Blanchfield L, Gerner EW, Mirmira RG, DiMeglio LA
Cell reports. Medicine 2023 Nov 21;4(11):101261

Sulfated fuco-manno-glucuronogalactan alleviates pancreatic beta cell senescence via PI3K/AKT/FoxO1 pathway.

Zhang W, Wu N, Wang H, Mao G, Yan X, Zhang F, Linhardt RJ, Jin W, Zhou J
International journal of biological macromolecules 2023 May 1;236:123846

Metabolomics analysis of islet regeneration in partial pancreatectomy mice reveals increased levels of long-chain fatty acids and activated cAMP signaling pathway.

Wu D, Jiang Y, Wang Z, Ni Y, Ma A, Zhou Y, Liu R, Lou YR, Wang Q
Biochemical and biophysical research communications 2023 Jul 30;667:34-42

Bacteroides ovatus accelerates metformin-induced vitamin B12 deficiency in type 2 diabetes patients by accumulating cobalamin.

Chen M, Shu Y, Li Q, Kang Z, Liu T, Zhou H, Huang W, Zhang W
NPJ biofilms and microbiomes 2023 Jul 24;9(1):51

Toceranib phosphate (Palladia) reverses type 1 diabetes by preserving islet function in mice.

Kishi K, Yonezawa T, Kaji N, Goto M, Nonoshita Y, Iio A, Tsuru Y, Hori M
The Journal of veterinary medical science 2023 Jul 17;85(7):781-789

Icariin ameliorates memory deficits through regulating brain insulin signaling and glucose transporters in 3×Tg-AD mice.

Yan F, Liu J, Chen MX, Zhang Y, Wei SJ, Jin H, Nie J, Fu XL, Shi JS, Zhou SY, Jin F
Neural regeneration research 2023 Jan;18(1):183-188

Combined therapy of GABA and sitagliptin prevents high-fat diet impairment of beta-cell function.

Wang Z, Fan L, Ni Y, Wu D, Ma A, Zhao Y, Li J, Cui Q, Zhou Y, Zhang L, Lou YR, Prud'homme GJ, Wang Q
Molecular and cellular endocrinology 2023 Jan 1;559:111755

Secretory autophagy promotes RAB37-mediated insulin secretion under glucose stimulation both in vitro and in vivo.

Wu SY, Wu HT, Wang YC, Chang CJ, Shan YS, Wu SR, Chiu YC, Hsu CL, Juan HF, Lan KY, Chu CW, Lee YR, Lan SH, Liu HS
Autophagy 2023 Apr;19(4):1239-1257

Experimental Periodontitis Deteriorates Cognitive Function and Impairs Insulin Signaling in a Streptozotocin-Induced Alzheimer's Disease Rat Model.

Duan L, Qian X, Wang Q, Huang L, Ge S
Journal of Alzheimer's disease : JAD 2022;88(1):57-74

Shenlian (SL) Decoction, a Traditional Chinese Medicine Compound, May Ameliorate Blood Glucose via Mediating the Gut Microbiota in db/db Mice.

Sun RX, Huang WJ, Xiao Y, Wang DD, Mu GH, Nan H, Ni BR, Huang XQ, Wang HC, Liu YF, Fu Q, Zhao JX
Journal of diabetes research 2022;2022:7802107

∆nFGF1 Protects β-Cells against High Glucose-Induced Apoptosis via the AMPK/SIRT1/PGC-1α Axis.

Chen Q, Chen X, Jia Z, Du Y, Zhang S, Xu W, Pan B, Lou J, Zhou J, Zhou J, Sun J
Oxidative medicine and cellular longevity 2022;2022:1231970

Cdc42 upregulation under high glucose induces podocyte apoptosis and impairs β-cell insulin secretion.

Jiang S, Xu CM, Yao S, Zhang R, Li XZ, Zhang RZ, Xie TY, Xing YQ, Zhang Q, Zhou XJ, Liao L, Dong JJ
Frontiers in endocrinology 2022;13:905703

The potential mechanism of Liu-Wei-Di-Huang Pills in treatment of type 2 diabetic mellitus: from gut microbiota to short-chain fatty acids metabolism.

Yi ZY, Chen L, Wang Y, He D, Zhao D, Zhang SH, Yu R, Huang JH
Acta diabetologica 2022 Oct;59(10):1295-1308

Type IIB PKA is highly expressed in β cells and controls cell proliferation via regulating Cyclin D1 expression.

Jiang Y, Zhu L, Wu D, Ni Y, Huang C, Ye H, Yang Y, Liu R, Li Y
The FEBS journal 2022 May;289(10):2865-2876

Isolation of mouse pancreatic islet Procr(+) progenitors and long-term expansion of islet organoids in vitro.

Wang J, Wang D, Chen X, Yuan S, Bai L, Liu C, Zeng YA
Nature protocols 2022 May;17(5):1359-1384

Deficiency of M-LP/Mpv17L leads to development of β-cell hyperplasia and improved glucose tolerance via activation of the Wnt and TGF-β pathways.

Iida R, Ueki M, Yasuda T
Biochimica et biophysica acta. Molecular basis of disease 2022 Mar 1;1868(3):166318

Stem Cell Therapy Improves Human Islet Graft Survival in Mice via Regulation of Macrophages.

Gou W, Hua W, Swaby L, Cui W, Green E, Morgan KA, Strange C, Wang H
Diabetes 2022 Dec 1;71(12):2642-2655

Engineering of hybrid spheroids of mesenchymal stem cells and drug depots for immunomodulating effect in islet xenotransplantation.

Nguyen TT, Pham DV, Park J, Phung CD, Nepal MR, Pandit M, Shrestha M, Son Y, Joshi M, Jeong TC, Park PH, Choi DY, Chang JH, Kim JH, Kim JR, Kim IK, Yong CS, Kim JO, Sung JH, Jiang HL, Kim HS, Yook S, Jeong JH
Science advances 2022 Aug 26;8(34):eabn8614

Melatonin Promotes the Therapeutic Effect of Mesenchymal Stem Cells on Type 2 Diabetes Mellitus by Regulating TGF-β Pathway.

Li B, Cheng X, Aierken A, Du J, He W, Zhang M, Tan N, Kou Z, Peng S, Jia W, Tang H, Hua J
Frontiers in cell and developmental biology 2021;9:722365

Neu-P11 - a novel melatonin receptor agonist, could improve the features of type-2 diabetes mellitus in rats.

Li X, He J, Li X, Li Y, Zhou Y, Cai S
Endokrynologia Polska 2021;72(6):634-642

JunD Regulates Pancreatic β-Cells Function by Altering Lipid Accumulation.

Wang K, Cui Y, Lin P, Yao Z, Sun Y
Frontiers in endocrinology 2021;12:689845

Meteorin-Like Ameliorates β Cell Function by Inhibiting β Cell Apoptosis of and Promoting β Cell Proliferation via Activating the WNT/β-Catenin Pathway.

Hu W, Wang R, Sun B
Frontiers in pharmacology 2021;12:627147

SNAPIN Regulates Cell Cycle Progression to Promote Pancreatic β Cell Growth.

Jiang M, Kuang Z, He Y, Cao Y, Yu T, Cheng J, Liu W, Wang W
Frontiers in endocrinology 2021;12:624309

Glucokinase is required for high-starch diet-induced β-cell mass expansion in mice.

Tsuchida K, Nakamura A, Miyoshi H, Yang K, Yamauchi Y, Kawata S, Omori K, Takahashi K, Kitao N, Nomoto H, Kameda H, Cho KY, Seino Y, Terauchi Y, Atsumi T
Journal of diabetes investigation 2021 Sep;12(9):1545-1554

HGF can reduce accumulation of inflammation and regulate glucose homeostasis in T2D mice.

Yan L, He X, Tang Y, Zhao X, Luo G, Wang X
Journal of physiology and biochemistry 2021 Nov;77(4):613-624

Mof acetyltransferase inhibition ameliorates glucose intolerance and islet dysfunction of type 2 diabetes via targeting pancreatic α-cells.

Guo X, Cui C, Song J, He Q, Zang N, Hu H, Wang X, Li D, Wang C, Hou X, Li X, Liang K, Yan F, Chen L
Molecular and cellular endocrinology 2021 Nov 1;537:111425

Morphological and biochemical changes in the pancreas associated with acute systemic hypoxia.

Morioka F, Tani N, Ikeda T, Hirokawa T, Ikeda K, Shida A, Aoki Y, Ishikawa T
Human cell 2021 Mar;34(2):400-418

12-Lipoxygenase governs the innate immune pathogenesis of islet inflammation and autoimmune diabetes.

Kulkarni A, Pineros AR, Walsh MA, Casimiro I, Ibrahim S, Hernandez-Perez M, Orr KS, Glenn L, Nadler JL, Morris MA, Tersey SA, Mirmira RG, Anderson RM
JCI insight 2021 Jul 22;6(14)

Nfe2l1 deficiency mitigates streptozotocin-induced pancreatic β-cell destruction and development of diabetes in male mice.

Bao S, Zheng H, Chen C, Zhang Y, Bao L, Yang B, Hou Y, Chen Y, Zhang Q, Pi J, Fu J
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2021 Dec;158:112633

Advanced glycation end products activated endothelial-to-mesenchymal transition in pancreatic islet endothelial cells and triggered islet fibrosis in diabetic mice.

Tsai PS, Chiu CY, Sheu ML, Yang CY, Lan KC, Liu SH
Chemico-biological interactions 2021 Aug 25;345:109562

Glucokinase Inactivation Paradoxically Ameliorates Glucose Intolerance by Increasing β-Cell Mass in db/db Mice.

Omori K, Nakamura A, Miyoshi H, Yamauchi Y, Kawata S, Takahashi K, Kitao N, Nomoto H, Kameda H, Cho KY, Terauchi Y, Atsumi T
Diabetes 2021 Apr;70(4):917-931

Circular RNA circPPM1F modulates M1 macrophage activation and pancreatic islet inflammation in type 1 diabetes mellitus.

Zhang C, Han X, Yang L, Fu J, Sun C, Huang S, Xiao W, Gao Y, Liang Q, Wang X, Luo F, Lu W, Zhou Y
Theranostics 2020;10(24):10908-10924

Depletion of the diabetic gut microbiota resistance enhances stem cells therapy in type 1 diabetes mellitus.

Lv W, Graves DT, He L, Shi Y, Deng X, Zhao Y, Dong X, Ren Y, Liu X, Xiao E, Zhang Y
Theranostics 2020;10(14):6500-6516

The MEK Inhibitor Trametinib Suppresses Major Histocompatibility Antigen-mismatched Rejection Following Pancreatic Islet Transplantation.

Tada S, Anazawa T, Shindo T, Yamane K, Inoguchi K, Fujimoto N, Nagai K, Masui T, Okajima H, Takaori K, Sumi S, Uemoto S
Transplantation direct 2020 Sep;6(9):e591

Tectorigenin enhances PDX1 expression and protects pancreatic β-cells by activating ERK and reducing ER stress.

Yao X, Li K, Liang C, Zhou Z, Wang J, Wang S, Liu L, Yu CL, Song ZB, Bao YL, Zheng LH, Sun Y, Wang G, Huang Y, Yi J, Sun L, Li Y
The Journal of biological chemistry 2020 Sep 11;295(37):12975-12992

Islet transplantation in the subcutaneous space achieves long-term euglycaemia in preclinical models of type 1 diabetes.

Yu M, Agarwal D, Korutla L, May CL, Wang W, Griffith NN, Hering BJ, Kaestner KH, Velazquez OC, Markmann JF, Vallabhajosyula P, Liu C, Naji A
Nature metabolism 2020 Oct;2(10):1013-1020

Long-Term Expansion of Pancreatic Islet Organoids from Resident Procr(+) Progenitors.

Wang D, Wang J, Bai L, Pan H, Feng H, Clevers H, Zeng YA
Cell 2020 Mar 19;180(6):1198-1211.e19

Localized Controlled Release of Bilirubin from β-Cyclodextrin-Conjugated ε-Polylysine To Attenuate Oxidative Stress and Inflammation in Transplanted Islets.

Yao Q, Huang ZW, Zhai YY, Yue M, Luo LZ, Xue PP, Han YH, Xu HL, Kou L, Zhao YZ
ACS applied materials & interfaces 2020 Feb 5;12(5):5462-5475

FABP4 inhibitor attenuates inflammation and endoplasmic reticulum stress of islet in leptin receptor knockout rats.

Yao F, Jiang DD, Guo WH, Guo LS, Gao MM, Bai Y, Wang X, Zhang LS
European review for medical and pharmacological sciences 2020 Dec;24(24):12808-12820

Mint3 is dispensable for pancreatic and kidney functions in mice.

Chung Y, Saitoh Y, Hayashi T, Fukui Y, Terada N, Seiki M, Murakami Y, Sakamoto T
Biochemistry and biophysics reports 2020 Dec;24:100872

Using Recombinant Human Collagen With Basic Fibroblast Growth Factor to Provide a Simulated Extracellular Matrix Microenvironment for the Revascularization and Attachment of Islets to the Transplantation Region.

Zhu Q, Lu C, Jiang X, Yao Q, Jiang X, Huang Z, Jiang Y, Peng L, Fu H, Zhao Y
Frontiers in pharmacology 2019;10:1536

TLR4 gene in the regulation of periodontitis and its molecular mechanism.

Qi W, Yang X, Ye N, Li S, Han Q, Huang J, Wu B
Experimental and therapeutic medicine 2019 Sep;18(3):1961-1966

Rotenone protects against β-cell apoptosis and attenuates type 1 diabetes mellitus.

Wu M, Chen W, Zhang S, Huang S, Zhang A, Zhang Y, Jia Z
Apoptosis : an international journal on programmed cell death 2019 Dec;24(11-12):879-891

Upregulation of vitamin D-binding protein is associated with changes in insulin production in pancreatic beta-cells exposed to p,p'-DDT and p,p'-DDE.

Pavlíková N, Daniel P, Šrámek J, Jelínek M, Šrámková V, Němcová V, Balušíková K, Halada P, Kovář J
Scientific reports 2019 Dec 2;9(1):18026

Salsalate Prevents β-Cell Dedifferentiation in OLETF Rats with Type 2 Diabetes through Notch1 Pathway.

Han F, Li X, Yang J, Liu H, Zhang Y, Yang X, Yang S, Chang B, Chen L, Chang B
Aging and disease 2019 Aug;10(4):719-730

Expression of P2X1 receptors in somatostatin-containing cells in mouse gastrointestinal tract and pancreatic islets of both mouse and human.

Ji R, Zhu J, Wang D, Sui QQ, Knight GE, Burnstock G, Yuan H, Xiang Z
Purinergic signalling 2018 Sep;14(3):285-298

AWRK6, a Novel GLP-1 Receptor Agonist, Attenuates Diabetes by Stimulating Insulin Secretion.

Wang Q, Zhao C, Jin L, Zhang H, Miao Q, Liu H, Zhang D
International journal of molecular sciences 2018 Oct 7;19(10)

The Synergy of Two Factors on Insulin Expression.

Luo Y, Xu Y, Wang ZY, Li X, Xing WB, Zhang TC
Cellular reprogramming 2018 Feb;20(1):49-54

Hepatitis B X-interacting protein promotes the formation of the insulin gene-transcribing protein complex Pdx-1/Neurod1 in animal pancreatic β-cells.

Li H, Wang Z, Li Y, Fang R, Wang H, Shi H, Zhang X, Zhang W, Ye L
The Journal of biological chemistry 2018 Feb 9;293(6):2053-2065

Differential protein analysis of serum exosomes post-intravenous immunoglobulin therapy in patients with Kawasaki disease.

Zhang L, Song QF, Jin JJ, Huang P, Wang ZP, Xie XF, Gu XQ, Gao XJ, Jia HL
Cardiology in the young 2017 Nov;27(9):1786-1796

Involvement of Insulin Signaling Disturbances in Bisphenol A-Induced Alzheimer's Disease-like Neurotoxicity.

Wang T, Xie C, Yu P, Fang F, Zhu J, Cheng J, Gu A, Wang J, Xiao H
Scientific reports 2017 Aug 8;7(1):7497

Secretory expression and surface display of a new and biologically active single-chain insulin (SCI-59) analog by lactic acid bacteria.

Mao R, Wu D, Hu S, Zhou K, Wang M, Wang Y
Applied microbiology and biotechnology 2017 Apr;101(8):3259-3271

The effect of cholesteryl ester transfer protein on pancreatic beta cell dysfunction in mice.

Guo W, Gong Y, Fu Z, Fu J, Sun Y, Ju X, Chang Y, Wang W, Zhu X, Gao B, Liu X, Yang T, Zhou H
Nutrition & metabolism 2016;13:21

Insulin signaling disruption in male mice due to perinatal bisphenol A exposure: Role of insulin signaling in the brain.

Fang F, Gao Y, Wang T, Chen D, Liu J, Qian W, Cheng J, Gao R, Wang J, Xiao H
Toxicology letters 2016 Mar 14;245:59-67

Bisphenol A disrupts glucose transport and neurophysiological role of IR/IRS/AKT/GSK3β axis in the brain of male mice.

Li J, Wang Y, Fang F, Chen D, Gao Y, Liu J, Gao R, Wang J, Xiao H
Environmental toxicology and pharmacology 2016 Apr;43:7-12

Silencing Mist1 Gene Expression Is Essential for Recovery from Acute Pancreatitis.

Karki A, Humphrey SE, Steele RE, Hess DA, Taparowsky EJ, Konieczny SF
PloS one 2015;10(12):e0145724

Effects of Bisphenol A on glucose homeostasis and brain insulin signaling pathways in male mice.

Fang F, Chen D, Yu P, Qian W, Zhou J, Liu J, Gao R, Wang J, Xiao H
General and comparative endocrinology 2015 Feb 1;212:44-50

CNC-bZIP protein Nrf1-dependent regulation of glucose-stimulated insulin secretion.

Zheng H, Fu J, Xue P, Zhao R, Dong J, Liu D, Yamamoto M, Tong Q, Teng W, Qu W, Zhang Q, Andersen ME, Pi J
Antioxidants & redox signaling 2015 Apr 1;22(10):819-31

Autoimmune diabetes is suppressed by treatment with recombinant human tissue Kallikrein-1.

Maneva-Radicheva L, Amatya C, Parker C, Ellefson J, Radichev I, Raghavan A, Charles ML, Williams MS, Robbins MS, Savinov AY
PloS one 2014;9(9):e107213

Display of human proinsulin on the Bacillus subtilis spore surface for oral administration.

Feng F, Hu P, Chen L, Tang Q, Lian C, Yao Q, Chen K
Current microbiology 2013 Jul;67(1):1-8

Tendons from non-diabetic humans and rats harbor a population of insulin-producing, pancreatic beta cell-like cells.

Lehner C, Gehwolf R, Wagner A, Resch H, Hirzinger C, Augat P, Stephan D, Aigner L, Rivera FJ, Bauer HC, Tempfer H
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme 2012 Jun;44(7):506-10

No comments: Submit comment

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15848-1-AP(INS antibody) at dilution of 1:10000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human pancreas using 15848-1-AP(INS antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The Insulin antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human Insulin. This antibody recognizes human,mouse,rat antigen. The Insulin antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 15848-1-AP.pdf

: Show more