Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 14-9769-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Insulin Monoclonal Antibody (ICBTACLS), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This ICBTACLS monoclonal antibody reacts with the hormone insulin, responsible for regulation of carbohydrate and fat metabolism. Blood glucose levels are maintained by insulin. Insulin secretion from pancreatic beta-cells within the islets of Langerhans results in uptake of excess glucose from the blood into organs and its storage in the form of glycogen. Patients with type 1 diabetes have an autoimmune destruction of insulin-producing beta-cells in the pancreas, resulting in insulin deficiency and elevated levels of blood glucose. Patients with type 2 diabetes have insulin resistance where insulin becomes less effective at reducing the amount of blood glucose. Insulin is also important for proper endothelial cell function in with respect to vascular elasticity in response to changing blood pressure and plays a role in cognition, learning, and memory. The ICBTACLS antibody recognizes human, mouse, and rat insulin. Applications Reported: This ICBTACLS antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy. Applications Tested: This ICBTACLS antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low or high pH antigen retrieval and can be used at less than or equal to 0.25 µg/mL. This ICBTACLS antibody has been tested by western blot of non-reduced pancreatic tissue lysate and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- ICBTACLS
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Effect of hTIMP-1 overexpression in human umbilical cord mesenchymal stem cells on the repair of pancreatic islets in type-1 diabetic mice.
Pregestational diet transition to normal-fat diet avoids the deterioration of pancreatic β-cell function in male offspring induced by maternal high-fat diet.
Black Seed Thymoquinone Improved Insulin Secretion, Hepatic Glycogen Storage, and Oxidative Stress in Streptozotocin-Induced Diabetic Male Wistar Rats.
Insulin in the brain: there and back again.
Type 1 diabetes: pathogenesis and prevention.
Bao Y, Zhao Z, Gao H
Cell biology international 2021 May;45(5):1038-1049
Cell biology international 2021 May;45(5):1038-1049
Pregestational diet transition to normal-fat diet avoids the deterioration of pancreatic β-cell function in male offspring induced by maternal high-fat diet.
Liu Z, Ding Z, Lynch EC, McCauley N, Zhou Y, Zhang KK, Xie L
The Journal of nutritional biochemistry 2020 Dec;86:108495
The Journal of nutritional biochemistry 2020 Dec;86:108495
Black Seed Thymoquinone Improved Insulin Secretion, Hepatic Glycogen Storage, and Oxidative Stress in Streptozotocin-Induced Diabetic Male Wistar Rats.
Abdelrazek HMA, Kilany OE, Muhammad MAA, Tag HM, Abdelazim AM
Oxidative medicine and cellular longevity 2018;2018:8104165
Oxidative medicine and cellular longevity 2018;2018:8104165
Insulin in the brain: there and back again.
Banks WA, Owen JB, Erickson MA
Pharmacology & therapeutics 2012 Oct;136(1):82-93
Pharmacology & therapeutics 2012 Oct;136(1):82-93
Type 1 diabetes: pathogenesis and prevention.
Gillespie KM
CMAJ : Canadian Medical Association journal = journal de l'Association medicale canadienne 2006 Jul 18;175(2):165-70
CMAJ : Canadian Medical Association journal = journal de l'Association medicale canadienne 2006 Jul 18;175(2):165-70
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 7 Figure Immunofluorescence staining of insulin and glucagon (x100). Green: insulin; red: glucagon; blue: DAPI. DAPI, 4',6-diamidino-2-phenylindole
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Immunohistochemical staining for insulin expression in pancreatic islets from different experimental groups. (a) Control, (b) NSO- (2 mL/kg) treated group, (c) diabetic group, and (d) diabetic group treated with NSO (2 mL/kg). Scale bar represents 50 mu m.