A700-084
antibody from Invitrogen Antibodies
Targeting: MED12
ARC240, CAGH45, FGS1, HOPA, KIAA0192, Kto, OKS, OPA1, TNRC11, TRAP230
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- A700-084 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MED12 Recombinant Rabbit Monoclonal Antibody (BLR084G)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- BLR084G
- Vial size
- 100 µL
- Concentration
- 250 µg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse MED12 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, A-549, MCF-7, Hep-G2, K-562, RKO, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MED12 recombinant monoclonal antibody [BLR084G] (Product # A700-084 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-eEF2 (Product # A301-688A).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MED12 in FFPE HCT-116 cells by ICC. Antibody: Rabbit anti-MED12 recombinant monoclonal antibody [BLR084G] (Product # A700-084; lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG (Product # A120-501P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MED12 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-MED12 recombinant monoclonal antibody (BLR084G) (A700-084 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse MED12 in FFPE renal carcinoma by IHC. Antibody: Rabbit anti-MED12 recombinant monoclonal antibody [BLR084G] (Product # A700-084 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MED12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-MED12 recombinant monoclonal antibody [BLR084G] (Product # A700-084 lot 1) used for IP at 20 µL/mg lysate. MED12 was also immunoprecipitated by rabbit anti-MED12 antibody (Product # A300-774A). For blotting immunoprecipitated MED12 (Product # A700-084) was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.