NBP1-86669

antibody from Novus Biologicals

Targeting: MED12 ARC240, CAGH45, FGS1, HOPA, KIAA0192, Kto, OKS, OPA1, TNRC11, TRAP230

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-86669

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-86669, RRID:AB_11016398

Product name

Rabbit Polyclonal MED12 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human MED12 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MED12 Antibody [NBP1-86669] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-MED12 antibody. Remaining relative intensity is presented.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669] - Staining of human pancreas shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669] - Staining of human placenta shows high expression.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669] - Staining in human placenta and pancreas tissues using anti-MED12 antibody. Corresponding MED12 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669] - Staining of human cerebral cortex, colon, spleen and testis using Anti-MED12 antibody NBP1-86669 (A) shows similar protein distribution across tissues to independent antibody NBP1-86670 (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669] - Staining of human spleen.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669] - Staining of human cerebral cortex.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669] - Staining of human testis.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669] - Staining of human colon.