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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- A302-192A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MINK1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 0.20 mg/mL
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Detection of human and mouse MINK1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-MINK1 antibody A302-192A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. MINK1 was also immunoprecipitated by rabbit anti-MINK1 antibodies A302-191A and A302-193A, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
