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- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- HPA023072 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA023072, RRID:AB_1846220
- Product name
- Anti-IDO1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human IDO1, Gene description: indoleamine 2,3-dioxygenase 1, Alternative Gene Names: IDO, INDO, Validated applications: WB, IHC, Uniprot ID: P14902, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Proteogenomic characterization of pancreatic neuroendocrine tumors uncovers hypoxia and immune signatures in clinically aggressive subtypes
Cannabinoid control of gingival immune activation in chronically SIV-infected rhesus macaques involves modulation of the indoleamine-2,3-dioxygenase-1 pathway and salivary microbiome
Evaluation of Novel Inhibitors of Tryptophan Dioxygenases for Enzyme and Species Selectivity Using Engineered Tumour Cell Lines Expressing Either Murine or Human IDO1 or TDO2
Immune-regulated IDO1-dependent tryptophan metabolism is source of one-carbon units for pancreatic cancer and stellate cells
Dysregulation at multiple points of the kynurenine pathway is a ubiquitous feature of renal cancer: implications for tumour immune evasion.
DNA methylation of indoleamine 2,3-dioxygenase 1 (IDO1) in head and neck squamous cell carcinomas correlates with IDO1 expression, HPV status, patients’ survival, immune cell infiltrates, mutational load, and interferon γ signature
Indoleamine 2,3-dioxygenase 1 and Programmed Cell Death-ligand 1 Co-expression Predicts Poor Pathologic Response and Recurrence in Esophageal Squamous Cell Carcinoma after Neoadjuvant Chemoradiotherapy
Granuloma Correlates of Protection Against Tuberculosis and Mechanisms of Immune Modulation by Mycobacterium tuberculosis
Tanaka A, Ogawa M, Zhou Y, Otani Y, Hendrickson R, Miele M, Li Z, Klimstra D, Wang J, Roehrl M
iScience 2024;27(8):110544
iScience 2024;27(8):110544
Cannabinoid control of gingival immune activation in chronically SIV-infected rhesus macaques involves modulation of the indoleamine-2,3-dioxygenase-1 pathway and salivary microbiome
McDew-White M, Lee E, Alvarez X, Sestak K, Ling B, Byrareddy S, Okeoma C, Mohan M
eBioMedicine 2022;75
eBioMedicine 2022;75
Evaluation of Novel Inhibitors of Tryptophan Dioxygenases for Enzyme and Species Selectivity Using Engineered Tumour Cell Lines Expressing Either Murine or Human IDO1 or TDO2
Tijono S, Palmer B, Tomek P, Flanagan J, Henare K, Gamage S, Braun L, Ching L
Pharmaceuticals 2022;15(9):1090
Pharmaceuticals 2022;15(9):1090
Immune-regulated IDO1-dependent tryptophan metabolism is source of one-carbon units for pancreatic cancer and stellate cells
Newman A, Falcone M, Huerta Uribe A, Zhang T, Athineos D, Pietzke M, Vazquez A, Blyth K, Maddocks O
Molecular Cell 2021;81(11):2290-2302.e7
Molecular Cell 2021;81(11):2290-2302.e7
Dysregulation at multiple points of the kynurenine pathway is a ubiquitous feature of renal cancer: implications for tumour immune evasion.
Hornigold N, Dunn KR, Craven RA, Zougman A, Trainor S, Shreeve R, Brown J, Sewell H, Shires M, Knowles M, Fukuwatari T, Maher ER, Burns J, Bhattarai S, Menon M, Brazma A, Scelo G, Feulner L, Riazalhosseini Y, Lathrop M, Harris A, Selby PJ, Banks RE, Vasudev NS
British journal of cancer 2020 Jul;123(1):137-147
British journal of cancer 2020 Jul;123(1):137-147
DNA methylation of indoleamine 2,3-dioxygenase 1 (IDO1) in head and neck squamous cell carcinomas correlates with IDO1 expression, HPV status, patients’ survival, immune cell infiltrates, mutational load, and interferon γ signature
Sailer V, Sailer U, Bawden E, Zarbl R, Wiek C, Vogt T, Dietrich J, Loick S, Grünwald I, Toma M, Golletz C, Gerstner A, Kristiansen G, Bootz F, Scheckenbach K, Landsberg J, Dietrich D
EBioMedicine 2019;48
EBioMedicine 2019;48
Indoleamine 2,3-dioxygenase 1 and Programmed Cell Death-ligand 1 Co-expression Predicts Poor Pathologic Response and Recurrence in Esophageal Squamous Cell Carcinoma after Neoadjuvant Chemoradiotherapy
Zhou S, Zhao L, Liang Z, Liu S, Li Y, Liu S, Yang H, Liu M, Xi M
Cancers 2019;11(2):169
Cancers 2019;11(2):169
Granuloma Correlates of Protection Against Tuberculosis and Mechanisms of Immune Modulation by Mycobacterium tuberculosis
Mehra S, Alvarez X, Didier P, Doyle L, Blanchard J, Lackner A, Kaushal D
The Journal of Infectious Diseases 2013;207(7):1115-1127
The Journal of Infectious Diseases 2013;207(7):1115-1127
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in EFO-21 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-IDO1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
- Sample type
- Human
- Protocol
- Protocol
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and IDO1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400784).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human placenta and cerebral cortex tissues using HPA023072 antibody. Corresponding IDO1 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
