TA506367

antibody from OriGene

Targeting: IDO1 IDO, INDO

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA506367

Provider

OriGene

Proper citation

OriGene Cat#TA506367, RRID:AB_2623730

Product name

IDO1 mouse monoclonal antibody, clone OTI2G4 (formerly 2G4)

Antibody type

Monoclonal

Description

IDO1 mouse monoclonal antibody, clone OTI2G4 (formerly 2G4)

Host

Mouse

Conjugate

Unconjugated

Epitope

IDO1

Isotype

IgG

Antibody clone number

OTI2G4

Vial size

100 µl

Concentration

NULL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDO1 (RC206592, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDO1.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IDO1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-IDO1 mouse monoclonal antibody (TA506367) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IDO1(RC206592).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-IDO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506367)

Validation comment

IHC