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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- TA506368 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA506368, RRID:AB_2623731
- Product name
- IDO1 mouse monoclonal antibody, clone OTI2B5 (formerly 2B5)
- Antibody type
- Monoclonal
- Description
- IDO1 mouse monoclonal antibody, clone OTI2B5 (formerly 2B5)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- IDO1
- Isotype
- IgG
- Antibody clone number
- OTI2B5
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDO1 (RC206592, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDO1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-IDO1 monoclonal antibody at 1:200.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-IDO1 mouse monoclonal antibody (TA506368) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IDO1(RC206592).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-IDO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506368)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-IDO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506368)
- Validation comment
- IHC
