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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [8]
- Other assay [1]
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- Product number
- UM500091 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM500091, RRID:AB_2629102
- Product name
- IDO1 mouse monoclonal antibody,clone UMAB126
- Antibody type
- Monoclonal
- Description
- IDO1 mouse monoclonal antibody,clone UMAB126
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- IDO1
- Isotype
- IgG
- Antibody clone number
- UMAB126
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
Submitted references Accumulation of an endogenous tryptophan-derived metabolite in colorectal and breast cancers.
Puccetti P, Fallarino F, Italiano A, Soubeyran I, MacGrogan G, Debled M, Velasco V, Bodet D, Eimer S, Veldhoen M, Prendergast GC, Platten M, Bessede A, Guillemin GJ
PloS one 2015;10(4):e0122046
PloS one 2015;10(4):e0122046
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Thyroid, 7: Colon, 8: Spleen 9: Liver, 10: Ovary). Diluation: 1:500.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of mouse tissue lysates (20ug) from Brian. Primary antibody diluation: 1:500. Secondary antibody dilution: Mouse TrueBlot? Ultra (1:1000).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of MCF-7 cells using anti-IDO1 mouse monoclonal antibody (UM500091, green, 1:100). Actin filaments were labeled with Alexa Fluor? 594 Phalloidin (red), and nuclear with DAPI (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-IDO1 mouse monoclonal antibody. (UM500091; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-IDO1 mouse monoclonal antibody. (UM500091; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-IDO1 mouse monoclonal antibody. (UM500091; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-IDO1 mouse monoclonal antibody. (UM500091; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human tonsil using anti-IDO1 mouse monoclonal antibody. (UM500091; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human placenta using anti-IDO1 clone UMAB126 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM500091 requires HIER with with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The placenta shows strong membrane and cytoplasmic in the endothelial cells.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human tonsil using anti-IDO1 clone UMAB126 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM500091 requires HIER with with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The tonsil shows strong membrane and cytoplasmic in the germinal center and rare strong nuclear, membrane, and cytoplasmic staining in the non germinal centers.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human spleen using anti-IDO1 clone UMAB126 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM500091 requires HIER with with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The spleen shows very few cells staining in the red pulp with strong nuclear, membrane, and cytoplasmic staining.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-IDO1 mouse monoclonal antibody (UM500091). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
- Validation comment
- 10K-CHIP
