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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB2571 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2571, RRID:AB_1575616
- Product name
- IDO1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of IDO1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Cytochrome b5, not superoxide anion radical, is a major reductant of indoleamine 2,3-dioxygenase in human cells.
The role of serine 167 in human indoleamine 2,3-dioxygenase: a comparison with tryptophan 2,3-dioxygenase.
Indoleamine 2,3-dioxygenase (IDO): the antagonist of type I interferon-driven skin inflammation?
Maghzal GJ, Thomas SR, Hunt NH, Stocker R
The Journal of biological chemistry 2008 May 2;283(18):12014-25
The Journal of biological chemistry 2008 May 2;283(18):12014-25
The role of serine 167 in human indoleamine 2,3-dioxygenase: a comparison with tryptophan 2,3-dioxygenase.
Chauhan N, Basran J, Efimov I, Svistunenko DA, Seward HE, Moody PC, Raven EL
Biochemistry 2008 Apr 22;47(16):4761-9
Biochemistry 2008 Apr 22;47(16):4761-9
Indoleamine 2,3-dioxygenase (IDO): the antagonist of type I interferon-driven skin inflammation?
Scheler M, Wenzel J, Tüting T, Takikawa O, Bieber T, von Bubnoff D
The American journal of pathology 2007 Dec;171(6):1936-43
The American journal of pathology 2007 Dec;171(6):1936-43
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of IDO1 polyclonal antibody (Cat # PAB2571) in 293 cell line lysates (35 ug/lane). IDO1 (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinomareacted with IDO1 polyclonal antibody (Cat # PAB2571), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
