IC6030G

antibody from R&D Systems

Targeting: IDO1 IDO, INDO

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC6030G

Provider

R&D Systems

Product name

Human Indoleamine 2,3-dioxygenase/IDO Alexa Fluor® 488-conjugated Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Indoleamine 2,3-dioxygenase/IDO in direct ELISAs.

Reactivity

Human

Host

Mouse

Conjugate

Green dye

Antigen sequence

P14902


Isotype

IgG

Antibody clone number

700838

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

References

Submitted references

Induction of indoleamine 2,3-dioxygenase by Borrelia burgdorferi in human immune cells correlates with pathogenic potential.

Love AC, Schwartz I, Petzke MM
Journal of leukocyte biology 2015 Feb;97(2):379-90

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CD4+ T cell-derived IL-21 and deprivation of CD40 signaling favor the in vivo development of granzyme B-expressing regulatory B cells in HIV patients.

Kaltenmeier C, Gawanbacht A, Beyer T, Lindner S, Trzaska T, van der Merwe JA, Härter G, Grüner B, Fabricius D, Lotfi R, Schwarz K, Schütz C, Hönig M, Schulz A, Kern P, Bommer M, Schrezenmeier H, Kirchhoff F, Jahrsdörfer B
Journal of immunology (Baltimore, Md. : 1950) 2015 Apr 15;194(8):3768-77

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Early growth response-2 signaling mediates immunomodulatory effects of human multipotential stromal cells.

Barbeau DJ, La KT, Kim DS, Kerpedjieva SS, Shurin GV, Tamama K
Stem cells and development 2014 Jan 15;23(2):155-66

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Indoleamine 2,3-dioxygenase/IDO in Human MDSCs by Flow Cytometry. Human myeloid-derived suppressor cells (MDSCs) treated with 10 ng/mL Recombinant Human IL-6 (Catalog # 206-IL) and 10 ng/mL Recombinant Human GM-CSF (Catalog # 215-GM) for 7 days were stained with Mouse Anti-Human Siglec-3/CD33 APC-conjugated Monoclonal Antibody (Catalog # FAB1137A) and either (A) Mouse Anti-Human Indoleamine 2,3-dioxygenase/IDO Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC6030G) or (B) Mouse IgG₁ Alexa Fluor 488 Isotype Control (Catalog # IC002G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.