IC6030P

antibody from R&D Systems

Targeting: IDO1 IDO, INDO

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC6030P

Provider

R&D Systems

Product name

Human Indoleamine 2,3-dioxygenase/IDO PE-conjugated Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Indoleamine 2,3-dioxygenase/IDO in direct ELISAs.

Reactivity

Human

Host

Mouse

Antigen sequence

P14902


Isotype

IgG

Antibody clone number

700838

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

References

Submitted references

Mesenchymal Stromal Cells Are More Immunosuppressive In Vitro If They Are Derived from Endometriotic Lesions than from Eutopic Endometrium.

Abomaray F, Gidlöf S, Götherström C
Stem cells international 2017;2017:3215962

---

Indoleamine 2, 3-dioxygenase (IDO) increases during renal fibrogenesis and its inhibition potentiates TGF-β 1-induced epithelial to mesenchymal transition.

Matheus LHG, Simão GM, Amaral TA, Brito RBO, Malta CS, Matos YST, Santana AC, Rodrigues GGC, Albejante MC, Bach EE, Dalboni MA, Camacho CP, Dellê H
BMC nephrology 2017 Sep 6;18(1):287

---

Type I interferon-mediated skewing of the serotonin synthesis is associated with severe disease in systemic lupus erythematosus.

Lood C, Tydén H, Gullstrand B, Klint C, Wenglén C, Nielsen CT, Heegaard NH, Jönsen A, Kahn R, Bengtsson AA
PloS one 2015;10(4):e0125109

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Indoleamine 2,3-dioxygenase/IDO in Human MDSCs by Flow Cytometry. Human myeloid-derived suppressor cells (MDSCs) treated with 10 ng/mL Recombinant Human IL-6 (Catalog # 206-IL) and 10 ng/mL Recombinant Human GM-CSF (Catalog # 215-GM) for 7 days were stained with Mouse Anti-Human Siglec-3/CD33 APC-conjugated Monoclonal Antibody (Catalog # FAB1137A) and either (A) Mouse Anti-Human Indoleamine 2,3-dioxygenase/IDO PE-conjugated Monoclonal Antibody (Catalog # IC6030P) or (B) Mouse IgG₁ Phycoerythrin Isotype Control (Catalog # IC002P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.