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LearnH00027344-M02
antibody from Abnova Corporation
Targeting: PCSK1N
BigLEN, PEN, proSAAS, SAAS, SCG8, SgVIII
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- H00027344-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00027344-M02, RRID:AB_1137353
- Product name
- PCSK1N monoclonal antibody (M02), clone 1E9
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant PCSK1N.
- Antigen sequence
DGPAGPDAEEAGDETPDVDPELLRYLLGRILAGSA
DSEGVAAPRRLRRAADHDVGSELPPEGVLGALLRV
KRLETPAPQVPARRLLPP- Isotype
- IgG
- Antibody clone number
- 1E9
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Pax6 directly down-regulates Pcsk1n expression thereby regulating PC1/3 dependent proinsulin processing.
Liu T, Zhao Y, Tang N, Feng R, Yang X, Lu N, Wen J, Li L
PloS one 2012;7(10):e46934
PloS one 2012;7(10):e46934
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PCSK1N expression in transfected 293T cell line by PCSK1N monoclonal antibody (M02), clone 1E9.Lane 1: PCSK1N transfected lysate (Predicted MW: 27.4 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PCSK1N is 0.1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to PCSK1N on HeLa cell . [antibody concentration 20 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to PCSK1N on formalin-fixed paraffin-embedded human cerebellum. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
