HPA023542

antibody from Atlas Antibodies

Targeting: APBB2 FE65L, FE65L1, MGC35575

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

HPA023542

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA023542, RRID:AB_1844909

Product name

Anti-APBB2

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human APBB2, Gene description: amyloid beta (A4) precursor protein-binding, family B, member 2, Alternative Gene Names: FE65L, FE65L1, MGC35575, Validated applications: ICC, IHC, WB, Uniprot ID: Q92870, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.2 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-APBB2 antibody. Remaining relative intensity is presented.

Sample type

Human

Protocol

Protocol

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and LY406673 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406673).

Sample type

Human

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG shows positivity in mitochondria.

Sample type

Human