Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-54816 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- APBB2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SEKLEGKEPH PQDSSSCEIL PSQPRRTKSF LNYYADLETS ARELEQNRGN HHGTAEEKSQ PVQGQASTII GNGDLLLQKP NRPQSSPEDG QVATVSSSPE TKKDHPKTGA KTDCALHRIQ NLAPS Highest antigen sequence identity to the following orthologs: Mouse - 85%, Rat - 23%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references miR‑205‑3p promotes lung cancer progression by targeting APBB2.
Xu LB, Xiong J, Zhang YH, Dai Y, Ren XP, Ren YJ, Han D, Wei SH, Qi M
Molecular medicine reports 2021 Aug;24(2)
Molecular medicine reports 2021 Aug;24(2)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of APBB2 in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a APBB2 Polyclonal Antibody (Product # PA5-54816). Remaining relative intensity is presented.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of APBB2 in control (vector only transfected HEK293T lysate) and LY406673 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a APBB2 Polyclonal Antibody (Product # PA5-54816).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of APBB2 in human cell line U-251 MG shows positivity in mitochondria. Samples were probed using an APBB2 Polyclonal Antibody (Product # PA5-54816).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of APBB2 in human pancreas shows distinct granular positivity in exocrine cells. Samples were probed using an APBB2 Polyclonal Antibody (Product # PA5-54816).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6. miR-205-3p antagomir inhibits lung cancer growth and promotes apoptosis in mouse xenografts. (A) Representative images of excised xenograft tumors and (B) tumor volumes from mice treated with the antagomir NC or miR-205-3p antagomir. (C) Reverse transcription-quantitative PCR analysis was used to detect miR-205-3p expression in tumors. (D) Western blot analysis was used to detect APBB2, Bax and Bcl-2 protein expression levels in tumors. *P