MA1-90652

antibody from Invitrogen Antibodies

Targeting: PLP1 GPM6C, PLP, SPG2

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

MA1-90652

Provider

Invitrogen Antibodies

Product name

PLP1 Monoclonal Antibody (plpc 1)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat, Bovine

Host

Mouse

Isotype

IgG

Antibody clone number

plpc 1

Vial size

50 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of PLP1 was performed using 70% confluent log phase Hep G2 and PC-3 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with PLP1 Monoclonal Antibody (plpc 1) (Product # MA1-90652) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723, 1:2000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization for PLP1 in Hep G2 cells. Panel e represents merged image of PC-3, which is reported to be negative for PLP1. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.