Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-37778 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-GAB2 (Tyr643) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control for Western blot is NIH-3T3 cells; suggested positive control for IHC is human brain tissue.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Time-resolved phosphoproteomics reveals scaffolding and catalysis-responsive patterns of SHP2-dependent signaling.
Vemulapalli V, Chylek LA, Erickson A, Pfeiffer A, Gabriel KH, LaRochelle J, Subramanian K, Cao R, Stegmaier K, Mohseni M, LaMarche MJ, Acker MG, Sorger PK, Gygi SP, Blacklow SC
eLife 2021 Mar 23;10
eLife 2021 Mar 23;10
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from NIH-3T3 cells (Lane 2) using Gab2 (pTyr643) polyclonal antibody (Product # PA5-37778) compared to the same antibody preincubated with a blocking peptide (Lane 1).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-GAB2 (Tyr643) in paraffin-embedded Human brain tissue using Phospho-GAB2 (Tyr643) Polyclonal Antibody (Product # PA5-37778) (left) or the same antibody preincubated with blocking peptide (right).