Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB2708 - Provider product page
- Provider
- R&D Systems
- Product name
- Human SR-AI/MSR Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human SR-AI/MSR in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse SR-AI is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P21757
- Isotype
- IgG
- Antibody clone number
- 351615
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Leptin modulates ACAT1 expression and cholesterol efflux from human macrophages.
Hongo S, Watanabe T, Arita S, Kanome T, Kageyama H, Shioda S, Miyazaki A
American journal of physiology. Endocrinology and metabolism 2009 Aug;297(2):E474-82
American journal of physiology. Endocrinology and metabolism 2009 Aug;297(2):E474-82
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Human SR-AI/MSR by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 80 nM PMA for 72 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SR-AI/MSR Monoclonal Antibody (Catalog # MAB2708) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SR-AI/MSR at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.