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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- 600-401-964 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-964, RRID:AB_2162300
- Product name
- Anti-Pdcd4 pS457 (RABBIT) Antibody - 600-401-964
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references Programmed cell death 4 loss increases tumor cell invasion and is regulated by miR-21 in oral squamous cell carcinoma.
Reis PP, Tomenson M, Cervigne NK, Machado J, Jurisica I, Pintilie M, Sukhai MA, Perez-Ordonez B, Grénman R, Gilbert RW, Gullane PJ, Irish JC, Kamel-Reid S
Molecular cancer 2010 Sep 10;9:238
Molecular cancer 2010 Sep 10;9:238
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's affinity purified anti-Pdcd4 pS457 antibody shows detection of Pdcd4 phosphorylated at Ser 457 (arrowheads). Lanes 1 & 2 each contain 100 ng recombinant Pdcd4. Lanes 3 & 4 each contain 30 µg of whole cell extract from 293 HEK cells treated with 20 nM TPA and MG132 proteosome inhibitor for 8 hours. The signal can be competed off with peptide phosphorylated at Ser 457 (Lanes 2 & 4). Personal Communication, M Young & A Jansen, NCI, Bethesda, MD.
- Validation comment
- Western Blot
