Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB4781 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4781, RRID:AB_1577345
- Product name
- MMP12 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of MMP12.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Crystal structure of human macrophage elastase (MMP-12) in complex with a hydroxamic acid inhibitor.
Substrate specificity determinants of human macrophage elastase (MMP-12) based on the 1.1 A crystal structure.
Hydrolysis of a broad spectrum of extracellular matrix proteins by human macrophage elastase.
Nar H, Werle K, Bauer MM, Dollinger H, Jung B
Journal of molecular biology 2001 Sep 28;312(4):743-51
Journal of molecular biology 2001 Sep 28;312(4):743-51
Substrate specificity determinants of human macrophage elastase (MMP-12) based on the 1.1 A crystal structure.
Lang R, Kocourek A, Braun M, Tschesche H, Huber R, Bode W, Maskos K
Journal of molecular biology 2001 Sep 28;312(4):731-42
Journal of molecular biology 2001 Sep 28;312(4):731-42
Hydrolysis of a broad spectrum of extracellular matrix proteins by human macrophage elastase.
Gronski TJ Jr, Martin RL, Kobayashi DK, Walsh BC, Holman MC, Huber M, Van Wart HE, Shapiro SD
The Journal of biological chemistry 1997 May 2;272(18):12189-94
The Journal of biological chemistry 1997 May 2;272(18):12189-94
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of MMP12 polyclonal antibody (Cat # PAB4781) in mouse kidney tissue lysate (35 ug/lane). MMP12 (arrow) was detected using the purified polyclonal antibody (1 : 240 dilution).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MMP12 polyclonal antibody (Cat # PAB4781) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)