PA5-13181

antibody from Invitrogen Antibodies

Targeting: MMP12 HME

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-13181

Provider

Invitrogen Antibodies

Product name

MMP12 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.35 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

PPARγ activation improves the microenvironment of perivascular adipose tissue and attenuates aortic stiffening in obesity.

Chen JY, Wu YP, Li CY, Jheng HF, Kao LZ, Yang CC, Leu SY, Lien IC, Weng WT, Tai HC, Chiou YW, Tang MJ, Tsai PJ, Tsai YS
Journal of biomedical science 2021 Mar 29;28(1):22

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of 293 cells using a MMP12 polyclonal antibody (Product # PA5-13181) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 4 Expression and location of elastolytic enzymes in the aorta and PVAT. Expression of genes for cathepsins and MMPs in the a aorta ( n = 7, 5, and 6 for control, ob/ob , and ob/ob + Piog groups respectively) and b PVAT ( n = 3, 5, and 6 for control, ob/ob , ob/ob + Piog groups respectively). mRNA amount is expressed relative to the average expression in control mice. * P < 0.05, ** P < 0.01, and *** P < 0.001. ND, not detectable. c Immunofluorescence staining for CTSS (green), MMP-12 (red) and F4/80 (blue) in the thoracic aorta and PVAT. The DAPI nuclear counterstain appears light blue. Magnification of the square in ( c ) is shown in ( d ). The while arrow indicates the location of elastic fiber break with increased signals of MMP-12 and F4/80. e Plasma levels of CTSS, MMP-12, and MCP-1 by ELISA (n = 7, 5, and 6 for control, ob/ob , and ob/ob + Piog groups respectively). All data are resulted from 3-month-old male mice. Original magnification x 400 for ( c ) and x 1200 for ( d )