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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-13181 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MMP12 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references PPARĪ³ activation improves the microenvironment of perivascular adipose tissue and attenuates aortic stiffening in obesity.
Chen JY, Wu YP, Li CY, Jheng HF, Kao LZ, Yang CC, Leu SY, Lien IC, Weng WT, Tai HC, Chiou YW, Tang MJ, Tsai PJ, Tsai YS
Journal of biomedical science 2021 Mar 29;28(1):22
Journal of biomedical science 2021 Mar 29;28(1):22
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of pre-incubated without (lane 1) and with (lane 2) blocking peptide in 293 cell lysate using a MMP12 polyclonal antibody (Product # PA5-13181).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of pre-incubated without (lane 1) and with (lane 2) blocking peptide in mouse kidney tissue lysates using a MMP12 polyclonal antibody (Product # PA5-13181).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 293 cells using a MMP12 polyclonal antibody (Product # PA5-13181) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue using a MMP12 polyclonal antibody (Product # PA5-13181), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 Expression and location of elastolytic enzymes in the aorta and PVAT. Expression of genes for cathepsins and MMPs in the a aorta ( n = 7, 5, and 6 for control, ob/ob , and ob/ob + Piog groups respectively) and b PVAT ( n = 3, 5, and 6 for control, ob/ob , ob/ob + Piog groups respectively). mRNA amount is expressed relative to the average expression in control mice. * P < 0.05, ** P < 0.01, and *** P < 0.001. ND, not detectable. c Immunofluorescence staining for CTSS (green), MMP-12 (red) and F4/80 (blue) in the thoracic aorta and PVAT. The DAPI nuclear counterstain appears light blue. Magnification of the square in ( c ) is shown in ( d ). The while arrow indicates the location of elastic fiber break with increased signals of MMP-12 and F4/80. e Plasma levels of CTSS, MMP-12, and MCP-1 by ELISA (n = 7, 5, and 6 for control, ob/ob , and ob/ob + Piog groups respectively). All data are resulted from 3-month-old male mice. Original magnification x 400 for ( c ) and x 1200 for ( d )
