Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF917 - Provider product page
- Provider
- R&D Systems
- Product name
- Human MMP-12 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human MMP-12 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse MMP-12 is observed, and less than 2% cross-reactivity with recombinant human (rh) MMP-1 and rhMMP-3 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P39900
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Morphological features of coronary plaques in WHHLMI rabbits (Oryctolagus cuniculus), an animal model for familial hypercholesterolemia.
Yamada S, Koike T, Nakagawa T, Kuniyoshi N, Ying Y, Itabe H, Yamashita A, Asada Y, Shiomi M
Experimental animals 2017 May 3;66(2):145-157
Experimental animals 2017 May 3;66(2):145-157
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Pro-MMP-12 by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human MMP-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF917) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Pro-MMP-12 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- MMP-12 in Human Squamous Cell Carcinoma. MMP-12 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Goat Anti-Human MMP-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF917) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.