PAB28591

antibody from Abnova Corporation

Targeting: APEX1 APE, APE-1, APEN, APEX, APX, HAP1, REF-1, REF1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28591

Provider

Abnova Corporation

Product name

APEX1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant APEX1.

Antigen sequence

KLPAELQELPGLSHQYWSAPSDKEGYSGVGLLSRQ
CPLKVSYGIGDEEHDQEGRVIVAEFDSFVLVTAYV
PNAGRGLVRLEYRQRWDEAFRKFLKGLASRKPLVL
CGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFGE
LLQAVPLAD


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Human cell line RT-4; Lane 2: Human cell line U-251MG sp; Lane 3: Human cell line A-431; Lane 4: Human liver tissue; Lane 6: Human tonsil tissue with APEX1 polyclonal antibody (Cat#PAB28591) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with APEX1 polyclonal antibody (Cat#PAB28591) at 4 ug/ml shows positivity in nucleus.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human small intestine with APEX1 polyclonal antibody (Cat#PAB28591) shows strong nuclear positivity in glandular cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)