TA800330

antibody from OriGene

Targeting: APEX1 APE, APE-1, APEN, APEX, APX, HAP1, REF-1, REF1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA800330

Provider

OriGene

Proper citation

OriGene Cat#TA800330, RRID:AB_2625381

Product name

APEX1 mouse monoclonal antibody, clone OTI1H9 (formerly 1H9)

Antibody type

Monoclonal

Description

APEX1 mouse monoclonal antibody, clone OTI1H9 (formerly 1H9)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

APEX1

Isotype

IgG

Antibody clone number

OTI1H9

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-APEX1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APEX1 (RC201732, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APEX1.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800330)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800330)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800330)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800330)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800330)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800330)

Validation comment

IHC