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LearnABIN2616817
antibody from antibodies-online
Targeting: APEX1
APE, APE-1, APEN, APEX, APX, HAP1, REF-1, REF1
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Chromatin Immunoprecipitation [1]
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- Product number
- ABIN2616817 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-APEX Nuclease (Multifunctional DNA Repair Enzyme) 1 (APEX1) (AA 32-318) antibody (Biotin)
- Antibody type
- Polyclonal
- Description
- Caprylic acid and ammonium sulfate precipitation
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Biotin
- Epitope
- AA 32-318
- Isotype
- IgG
- Vial size
- 100 μg
- Storage
- Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
- Handling
- Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Histone H4 acetyl Lys16 antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H4 acetyl Lys16 antibody for acetyl Lys16 histone H4. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H4 acetyl Lys16 antibody at a dilution of 1 μg/ml. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: acetyl-Lys5 peptide. Lane 2: unmodified Lys5 peptide. Lane 3: acetyl-Lys8 peptide. Lane 4: unmodified Lys8 peptide. Lane 5: acetyl-Lys12 peptide. Lane 6: unmodified Lys12 peptide. Lane 7: acetyl-Lys16 peptide. Lane 8: unmodified Lys16 peptide.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Histone H4 acetyl Lys16 antibody (pAb) tested by Western blot. HeLa nuclear extract (20 μg per lane) probed with Histone H4 acetyl Lys16 antibody (1 μg per ml). Lane 1: no treatment. Lane 2: cells treated with sodium butyrate.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Histone H4 acetyl Lys16 antibody tested by ChIP analysis. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 μg of Histone H4 acetyl Lys16 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
