PAB6125
antibody from Abnova Corporation
Targeting: APEX1
APE, APE-1, APEN, APEX, APX, HAP1, REF-1, REF1
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
Comment
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- Product number
- PAB6125 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6125, RRID:AB_1571249
- Product name
- APEX1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of APEX1.
- Antigen sequence
PKRGKKGAVAEDGD-C
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Cloning and expression of APE, the cDNA encoding the major human apurinic endonuclease: definition of a family of DNA repair enzymes.
Demple B, Herman T, Chen DS
Proceedings of the National Academy of Sciences of the United States of America 1991 Dec 15;88(24):11450-4
Proceedings of the National Academy of Sciences of the United States of America 1991 Dec 15;88(24):11450-4
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- APEX1 polyclonal antibody (Cat # PAB6125) staining (0.1 ug/mL) of A-431 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- APEX1 polyclonal antibody (Cat # PAB6125) (4 ug/mL) staining of paraffin embedded human breast. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)