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LearnH00000328-M05
antibody from Abnova Corporation
Targeting: APEX1
APE, APE-1, APEN, APEX, APX, HAP1, REF-1, REF1
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00000328-M05 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000328-M05, RRID:AB_10717696
- Product name
- APEX1 monoclonal antibody (M05), clone 3E12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant APEX1.
- Antigen sequence
MPKRGKKGAVAEDGDELRTEPEAKKSKTAAKKNDK
EAAGEGPALYEDPPDQKTSPSGKPATLKICSWNVD
GLRAWIKKKGLDWVKEEAPDILCLQETKCSENKLP
AELQELPGLSHQYWSAPSDKEGYSGVGLLSRQCPL
KVSYGIGEEEHDQEGRVIVAEFDSFVLVTAYVPNA
GRGLVRLEYRQRWDEAFRKFLKGLASRKPLVLCGD
LNVAHEEIDLRNPKGNKKNAGFTPQERQGFGELLQ
AVPLADSFRHLYPNTPYAYTFWTYMMNARSKNVGW
RLDYFLLSHSLLPALCDSKIRSKALGSDHCPITLY
LAL- Isotype
- IgG
- Antibody clone number
- 3E12
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged APEX1 is 0.1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of APEX1 transfected lysate using anti-APEX1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with APEX1 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
