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LearnPA1-9007
antibody from Invitrogen Antibodies
Targeting: APEX1
APE, APE-1, APEN, APEX, APX, HAP1, REF-1, REF1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA1-9007 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- APE1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, canine and porcine based on sequence homology.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of APE1 by a APE1 monoclonal antibody (Product # PA1-9007) at a concentration of 0.3 µg/mL. A431 (A), HeLa (B) and MCF7 (C) nuclear lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 illustrates Western blot detection of APEX1 in A431 lysate using Product # PA1-9007.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of APE1 in Human Breast using a APE1 monoclonal antibody (Product #PA1-9007) at 4 µg/mL. The Human Breast tissue section was paraffin embeded and detected using steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
