LS-C359295

antibody from LSBio

Targeting: NLRP3 AGTAVPRL, AII, AVP, C1orf7, CIAS1, CLR1.1, DFNA34, FCAS, FCU, MWS, NALP3, PYPAF1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C359295

Provider

LSBio

Product name

NALP3 / NLRP3 Antibody (aa216-385, clone 25N10E9) LS-C359295

Antibody type

Monoclonal

Description

Protein G purified

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

25N10E9

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot: NALP3 Antibody (25N10E9) - Western blot analysis of Raw 264 cell lysate using NALP3 antibody at 2 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot: NALP3 Antibody (25N10E9) - Analysis using the NALP3 monoclonal antibody. (A), (B), mouse RAW lysate and the recombinant mouse NLRP3 NOD domain protein immunogen (D) probed with 2 ug/ml antibody. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot: NLRP3/NALP3 Antibody (25N10E9) - Western blot shows a specific band for NALP3 in 0.5 mg/ml of RAW lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. This image was taken for the unconjugated form of this product. Other forms have not been tested.