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LearnMA5-32255
antibody from Invitrogen Antibodies
Targeting: NLRP3
AGTAVPRL, AII, AVP, C1orf7, CIAS1, CLR1.1, DFNA34, FCAS, FCU, MWS, NALP3, PYPAF1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [4]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [2]
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- Product number
- MA5-32255 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NLRP3 Recombinant Rabbit Monoclonal Antibody (SC06-23)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- SC06-23
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Oncostatin M promotes the ox-LDL-induced activation of NLRP3 inflammasomes via the NF-κB pathway in THP-1 macrophages and promotes the progression of atherosclerosis.
Ultrashort Wave Combined with Human Umbilical Cord Mesenchymal Stem Cell (HUC-MSC) Transplantation Inhibits NLRP3 Inflammasome and Improves Spinal Cord Injury via MK2/TTP Signalling Pathway.
Liu C, Wu J, Jia H, Lu C, Liu J, Li Y, Guo M
Annals of translational medicine 2022 Apr;10(8):456
Annals of translational medicine 2022 Apr;10(8):456
Ultrashort Wave Combined with Human Umbilical Cord Mesenchymal Stem Cell (HUC-MSC) Transplantation Inhibits NLRP3 Inflammasome and Improves Spinal Cord Injury via MK2/TTP Signalling Pathway.
Na L, Wang S, Liu T, Zhang L
BioMed research international 2020;2020:3021750
BioMed research international 2020;2020:3021750
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-NLRP3 Recombinant Rabbit Monoclonal Antibody (SC06-23) (Product # MA5-32255) and a 106 kDa band corresponding to Angiotensin/vasopressin receptor AII/AVP-like; AVP; was observed across cell lines tested. Whole cell extracts (30 µg lysate) of HUVEC (Lane 1), U-937 (Lane 2), RAW 264.7 (Lane 3), THP-1 (Lane 4) and K-562 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX), 10 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:3000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20,000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of NLRP3 in Hela cells using a NLRP3 Monoclonal antibody (Product # MA5-32255) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of NLRP3 in HUVEC cells using a NLRP3 Monoclonal antibody (Product # MA5-32255) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of NLRP3 in PMVEC cells using a NLRP3 Monoclonal antibody (Product # MA5-32255) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Angiotensin/vasopressin receptor AII/AVP-like; AVP; was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with NLRP3 Recombinant Rabbit Monoclonal Antibody (SC06-23) (Product # MA5-32255) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790, 1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing Cytosolic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X with oil immersion magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NLRP3 of paraffin-embedded Mouse bladder tissue using a NLRP3 Monoclonal antibody (Product #MA5-32255). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NLRP3 of paraffin-embedded Mouse spleen tissue using a NLRP3 Monoclonal antibody (Product #MA5-32255). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of NLRP3 in Jurkat cells using a NLRP3 Monoclonal Antibody (Product # MA5-32255) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Silencing OSM inhibits the ox-LDL-induced p65-NLRP3 pathway. THP-1 macrophages transfected with si-NC or OSM siRNAs (si-OSM-1 and si-OSM-2) were treated with 40 ug/mL ox-LDL for 48 hours. Protein expression of p65 and NLRP3 was analyzed by Western blotting (A). NLRP3 expression was analyzed by immunofluorescence (magnification, x400) (B). Protein expression of cleaved caspase-1, ASC, and GSDMD-N was analyzed by Western blotting (C). ASC, apoptosis-associated, speck-like protein containing a caspase-1 recruitment domain; GSDMD-N, gasdermin-D-N; NC, negative control; NLRP3, NLR family pyrin domain containing 3; OSM, oncostatin M; ox-LDL, oxidized low-density lipoprotein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Silencing OSM inhibits foam cell formation via the inhibition of NLRP3. THP-1 macrophages transfected with OSM siRNA (si-OSM-2) alone or in combination with pcNLRP3 were treated with 40 ug/mL ox-LDL for 48 hours. The mRNA (A) and protein (B) levels of NLRP3 were measured by qRT-PCR and Western blotting, respectively. Lipid accumulation (C) and total cholesterol content (D) were measured by Oil Red O staining and a commercial kit, respectively. Scale bars =20 um. n=3. Data presented as mean +- SD. Statistical analysis was performed by ANOVA. *, P
