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antibody from Invitrogen Antibodies
Targeting: NLRP3
AGTAVPRL, AII, AVP, C1orf7, CIAS1, CLR1.1, DFNA34, FCAS, FCU, MWS, NALP3, PYPAF1
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Flow cytometry [1]
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- Product number
- PA5-109211 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-NLRP3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: Human lung tissue lysate, mouse thymus tissue lysate, THP-1, A431, HepG2, LOVO, human liver cancer tissue, human colon cancer tissue, human placenta tissue, mouse brain tissue, mouse kidney tissue, Jurkat.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NLRP3 in Lane 1: Human lung, Lane 2: Mouse thymus, Lane 3: THP-1. Samples were incubated with NLRP3 polyclonal antibody (Product # PA5-109211), at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NLRP3 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with NLRP3 polyclonal antibody (Product # PA5-109211), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NLRP3 in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with NLRP3 polyclonal antibody (Product # PA5-109211), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NLRP3 in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with NLRP3 polyclonal antibody (Product # PA5-109211), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of NLRP3 in Jurkat cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with NLRP3 polyclonal antibody (Product # PA5-109211) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
