PA5-20838

antibody from Invitrogen Antibodies

Targeting: NLRP3 AGTAVPRL, AII, AVP, C1orf7, CIAS1, CLR1.1, DFNA34, FCAS, FCU, MWS, NALP3, PYPAF1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-20838

Provider

Invitrogen Antibodies

Product name

NLRP3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control is K562 cell lysate. PA5-20838 can be used with blocking peptide PEP-0952.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

References

Submitted references

Modafinil Reduces Neuronal Pyroptosis and Cognitive Decline After Sleep Deprivation.

Xiong X, Zuo Y, Cheng L, Yin Z, Hu T, Guo M, Han Z, Ge X, Li W, Wang Y, Wang D, Wang C, Zhang L, Zhang Y, Liu Q, Chen F, Lei P
Frontiers in neuroscience 2022;16:816752

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Malaria parasite heme biosynthesis promotes and griseofulvin protects against cerebral malaria in mice.

Chandana M, Anand A, Ghosh S, Das R, Beura S, Jena S, Suryawanshi AR, Padmanaban G, Nagaraj VA
Nature communications 2022 Jul 12;13(1):4028

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Role of Surfactant Protein D in Experimental Otitis Media.

Abdel-Razek O, Liu T, Chen X, Wang Q, Vanga G, Wang G
Journal of innate immunity 2021;13(4):197-210

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TRIM31 inhibits NLRP3 inflammasome and pyroptosis of retinal pigment epithelial cells through ubiquitination of NLRP3.

Huang P, Liu W, Chen J, Hu Y, Wang Y, Sun J, Feng J
Cell biology international 2020 Nov;44(11):2213-2219

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Surfactant Protein D Dampens Lung Injury by Suppressing NLRP3 Inflammasome Activation and NF-κB Signaling in Acute Pancreatitis.

Yu J, Ni L, Zhang X, Zhang J, Abdel-Razek O, Wang G
Shock (Augusta, Ga.) 2019 May;51(5):557-568

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of K562 cell lysate using a NALP3 polyclonal antibody (Product # PA5-20838) at 1 µg/mL in either (A) the presence and (B) the absence of blocking peptide.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of NALP3 in K562 cell lysate with NLRP3 Polyclonal Antibody (Product # PA5-20838) at 1 µg/mL (A) in the absence and (B) in the presence of blocking peptide.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of K562 cells using a NALP3 polyclonal antibody (Product # PA5-20838) at a 20 µg/mL dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry of NALP3 in K562 cells with NLRP3 Polyclonal Antibody (Product # PA5-20838) at 2 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of NALP3 in K562 cells with NLRP3 Polyclonal Antibody (Product # PA5-20838) at 20 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of NALP3 in K562 cells with NLRP3 Polyclonal Antibody (Product # PA5-20838) at 5 µg/mL. Red: NALP3 Blue: DAPI staining

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

4 Figure Effect of TRIM31 overexpression on NLRP3 ubiquitination. (a) Confirmation of the interactions between TRIM31 and NLRP3 by coimmunoprecipitation and western blot analysis in human RPE cells. (b) ARPE-19 cells were transfected with vector or plasmid overexpressing TRIM31 (oeTRIM31) for 48 hr and subjected to immunoprecipitation experiments. Anti-ubiquitin (Ub) detected NLRP3 ubiquitination in each group. IgG, immunoglobulin G; RPE, retinal pigment epithelial

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 3 Pyroptosis induced by SD was inhibited by modafinil. (A,B) Western blot data showing that modafinil suppressed the increase in the expression of NLRP3, NLRC4, NLRP1, ASC, caspase-1, GSDMD, and the downstream pro-inflammatory cytokines IL-1beta and IL-18 in mice subjected to SD. All data presented are means +- SD; N = 4-5 mice per group. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.