NBP2-12446

antibody from Novus Biologicals

Targeting: NLRP3 AGTAVPRL, AII, AVP, C1orf7, CIAS1, CLR1.1, DFNA34, FCAS, FCU, MWS, NALP3, PYPAF1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-12446

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP2-12446, RRID:AB_2750946

Product name

Rabbit Polyclonal NLRP3/NALP3 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.2 ml

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Anti-hyperglycemic activity of myricetin, through inhibition of DPP-4 and enhanced GLP-1 levels, is attenuated by co-ingestion with lectin-rich protein.

Lalitha N, Sadashivaiah B, Ramaprasad TR, Singh SA
PloS one 2020;15(4):e0231543

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Expression profiling of spinal cord dorsal horn in a rat model of complex regional pain syndrome type-I uncovers potential mechanisms mediating pain and neuroinflammation responses.

Chen R, Yin C, Hu Q, Liu B, Tai Y, Zheng X, Li Y, Fang J, Liu B
Journal of neuroinflammation 2020 May 23;17(1):162

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Jmjd3 regulates inflammasome activation and aggravates DSS-induced colitis in mice.

Huang M, Wang Q, Long F, Di Y, Wang J, Zhun Zhu Y, Liu X
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Mar;34(3):4107-4119

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MMP9 mediates acute hyperglycemia-induced human cardiac stem cell death by upregulating apoptosis and pyroptosis in vitro.

Yadav SK, Kambis TN, Kar S, Park SY, Mishra PK
Cell death & disease 2020 Mar 13;11(3):186

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Glyburide inhibits the bone resorption induced by traumatic occlusion in rats.

Arita Y, Yoshinaga Y, Kaneko T, Kawahara Y, Nakamura K, Ohgi K, Arita S, Ryu T, Takase M, Sakagami R
Journal of periodontal research 2020 Jun;55(3):464-471

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High-Mobility Group Box 1 Protein Signaling in Painful Diabetic Neuropathy.

Thakur V, Sadanandan J, Chattopadhyay M
International journal of molecular sciences 2020 Jan 30;21(3)

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Exendin-4 Protects against Hyperglycemia-Induced Cardiomyocyte Pyroptosis via the AMPK-TXNIP Pathway.

Wei H, Bu R, Yang Q, Jia J, Li T, Wang Q, Chen Y
Journal of diabetes research 2019;2019:8905917

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Emodin alleviates myocardial ischemia/reperfusion injury by inhibiting gasdermin D-mediated pyroptosis in cardiomyocytes.

Ye B, Chen X, Dai S, Han J, Liang X, Lin S, Cai X, Huang Z, Huang W
Drug design, development and therapy 2019;13:975-990

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Silencing of A20 Aggravates Neuronal Death and Inflammation After Traumatic Brain Injury: A Potential Trigger of Necroptosis.

Bao Z, Fan L, Zhao L, Xu X, Liu Y, Chao H, Liu N, You Y, Liu Y, Wang X, Ji J
Frontiers in molecular neuroscience 2019;12:222

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NLRP3 Inflammasome Mediated Interleukin-1β Production in Cancer-Associated Fibroblast Contributes to ALA-PDT for Cutaneous Squamous Cell Carcinoma.

Nie S, Wang X, Wang H
Cancer management and research 2019;11:10257-10267

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Inflammasome Formation in Granulomas in Cardiac Sarcoidosis.

Kron J, Mauro AG, Bonaventura A, Toldo S, Salloum FN, Ellenbogen KA, Abbate A
Circulation. Arrhythmia and electrophysiology 2019 Sep;12(9):e007582

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NLRP3 inflammasome activation in platelets in response to sepsis.

Cornelius DC, Baik CH, Travis OK, White DL, Young CM, Austin Pierce W, Shields CA, Poudel B, Williams JM
Physiological reports 2019 May;7(9):e14073

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TRPV1 mediates astrocyte activation and interleukin-1β release induced by hypoxic ischemia (HI).

Yang XL, Wang X, Shao L, Jiang GT, Min JW, Mei XY, He XH, Liu WH, Huang WX, Peng BW
Journal of neuroinflammation 2019 May 29;16(1):114

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Oral Administration of Compound Probiotics Ameliorates HFD-Induced Gut Microbe Dysbiosis and Chronic Metabolic Inflammation via the G Protein-Coupled Receptor 43 in Non-alcoholic Fatty Liver Disease Rats.

Liang Y, Liang S, Zhang Y, Deng Y, He Y, Chen Y, Liu C, Lin C, Yang Q
Probiotics and antimicrobial proteins 2019 Mar;11(1):175-185

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Gasdermin D serves as a key executioner of pyroptosis in experimental cerebral ischemia and reperfusion model both in vivo and in vitro.

Zhang D, Qian J, Zhang P, Li H, Shen H, Li X, Chen G
Journal of neuroscience research 2019 Jun;97(6):645-660

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Zhen-wu-tang ameliorates membranous nephropathy rats through inhibiting NF-κB pathway and NLRP3 inflammasome.

Liu B, Lu R, Li H, Zhou Y, Zhang P, Bai L, Chen D, Chen J, Li J, Yu P, Wu J, Liang C, Song J, Liu X, Zhou J
Phytomedicine : international journal of phytotherapy and phytopharmacology 2019 Jun;59:152913

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Milk Fat Globule Membrane Inhibits NLRP3 Inflammasome Activation and Enhances Intestinal Barrier Function in a Rat Model of Short Bowel.

Li Y, Wu J, Niu Y, Chen H, Tang Q, Zhong Y, Lambers TT, Cai W
JPEN. Journal of parenteral and enteral nutrition 2019 Jul;43(5):677-685

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Involvement of the microglial NLRP3 inflammasome in the anti-inflammatory effect of the antidepressant clomipramine.

Gong W, Zhang S, Zong Y, Halim M, Ren Z, Wang Y, Ma Y, Li B, Ma L, Zhou G, Yu J, Zhang J, Liu Q
Journal of affective disorders 2019 Jul 1;254:15-25

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Involvement of inflammasome activation via elevation of uric acid level in nociception in a mouse model of muscle pain.

Yoshida S, Hagiwara Y, Tsuchiya M, Shinoda M, Koide M, Hatakeyama H, Chaweewannakorn C, Suzuki K, Yano T, Sogi Y, Itaya N, Sekiguchi T, Yabe Y, Sasaki K, Kanzaki M, Itoi E
Molecular pain 2019 Jan-Dec;15:1744806919858797

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C1q/TNF-related protein-9 attenuates atherosclerosis through AMPK-NLRP3 inflammasome singling pathway.

Zhang H, Gong X, Ni S, Wang Y, Zhu L, Ji N
International immunopharmacology 2019 Dec;77:105934

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Exercise Training Promotes Cardiac Hydrogen Sulfide Biosynthesis and Mitigates Pyroptosis to Prevent High-Fat Diet-Induced Diabetic Cardiomyopathy.

Kar S, Shahshahan HR, Hackfort BT, Yadav SK, Yadav R, Kambis TN, Lefer DJ, Mishra PK
Antioxidants (Basel, Switzerland) 2019 Dec 11;8(12)

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Activation of Sphingosine-1-Phosphate Receptor 1 in the Spinal Cord Produces Mechanohypersensitivity Through the Activation of Inflammasome and IL-1β Pathway.

Doyle TM, Chen Z, Durante M, Salvemini D
The journal of pain 2019 Aug;20(8):956-964

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Amyloid β-dependent mitochondrial toxicity in mouse microglia requires P2X7 receptor expression and is prevented by nimodipine.

Chiozzi P, Sarti AC, Sanz JM, Giuliani AL, Adinolfi E, Vultaggio-Poma V, Falzoni S, Di Virgilio F
Scientific reports 2019 Apr 24;9(1):6475

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IRAK-M Deficiency Exacerbates Ischemic Neurovascular Injuries in Experimental Stroke Mice.

Lyu C, Zhang Y, Gu M, Huang Y, Liu G, Wang C, Li M, Chen S, Pan S, Gu Y
Frontiers in cellular neuroscience 2018;12:504

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Microbial metabolite sensor GPR43 controls severity of experimental GVHD.

Fujiwara H, Docampo MD, Riwes M, Peltier D, Toubai T, Henig I, Wu SJ, Kim S, Taylor A, Brabbs S, Liu C, Zajac C, Oravecz-Wilson K, Sun Y, Núñez G, Levine JE, van den Brink MRM, Ferrara JLM, Reddy P
Nature communications 2018 Sep 10;9(1):3674

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Andrographolide ameliorates intracerebral hemorrhage induced secondary brain injury by inhibiting neuroinflammation induction.

Li X, Wang T, Zhang D, Li H, Shen H, Ding X, Chen G
Neuropharmacology 2018 Oct;141:305-315

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Regulation of the NLRP3 inflammasome and macrophage pyroptosis by the p38 MAPK signaling pathway in a mouse model of acute lung injury.

Li D, Ren W, Jiang Z, Zhu L
Molecular medicine reports 2018 Nov;18(5):4399-4409

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Purinergic 2X7 Receptor is Involved in the Podocyte Damage of Obesity-Related Glomerulopathy via Activating Nucleotide-Binding and Oligomerization Domain-Like Receptor Protein 3 Inflammasome.

Hou XX, Dong HR, Sun LJ, Yang M, Cheng H, Chen YP
Chinese medical journal 2018 Nov 20;131(22):2713-2725

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NLRP3 Inflammasome Inhibitor Ameliorates Amyloid Pathology in a Mouse Model of Alzheimer's Disease.

Yin J, Zhao F, Chojnacki JE, Fulp J, Klein WL, Zhang S, Zhu X
Molecular neurobiology 2018 Mar;55(3):1977-1987

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Uric acid upregulates the adiponectin‑adiponectin receptor 1 pathway in renal proximal tubule epithelial cells.

Yang Q, Fu C, Xiao J, Ye Z
Molecular medicine reports 2018 Mar;17(3):3545-3554

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Mangiferin suppresses endoplasmic reticulum stress in perivascular adipose tissue and prevents insulin resistance in the endothelium.

Xu X, Chen Y, Song J, Hou F, Ma X, Liu B, Huang F
European journal of nutrition 2018 Jun;57(4):1563-1575

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Issues with the Specificity of Immunological Reagents for NLRP3: Implications for Age-related Macular Degeneration.

Kosmidou C, Efstathiou NE, Hoang MV, Notomi S, Konstantinou EK, Hirano M, Takahashi K, Maidana DE, Tsoka P, Young L, Gragoudas ES, Olsen TW, Morizane Y, Miller JW, Vavvas DG
Scientific reports 2018 Jan 11;8(1):461

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Lipid environment induces ER stress, TXNIP expression and inflammation in immune cells of individuals with type 2 diabetes.

Szpigel A, Hainault I, Carlier A, Venteclef N, Batto AF, Hajduch E, Bernard C, Ktorza A, Gautier JF, Ferré P, Bourron O, Foufelle F
Diabetologia 2018 Feb;61(2):399-412

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Paeonia lactiflora Pall. regulates the NF-κB-NLRP3 inflammasome pathway to alleviate cholestasis in rats.

Ma X, Wen JX, Gao SJ, He X, Li PY, Yang YX, Wei SZ, Zhao YL, Xiao XH
The Journal of pharmacy and pharmacology 2018 Dec;70(12):1675-1687

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Subretinal macrophages produce classical complement activator C1q leading to the progression of focal retinal degeneration.

Jiao H, Rutar M, Fernando N, Yednock T, Sankaranarayanan S, Aggio-Bruce R, Provis J, Natoli R
Molecular neurodegeneration 2018 Aug 20;13(1):45

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Perilla aldehyde attenuates CUMS-induced depressive-like behaviors via regulating TXNIP/TRX/NLRP3 pathway in rats.

Song Y, Sun R, Ji Z, Li X, Fu Q, Ma S
Life sciences 2018 Aug 1;206:117-124

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Pioglitazone ameliorates glomerular NLRP3 inflammasome activation in apolipoprotein E knockout mice with diabetes mellitus.

Wang Y, Yu B, Wang L, Yang M, Xia Z, Wei W, Zhang F, Yuan X
PloS one 2017;12(7):e0181248

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Long-term administration of advanced glycation end-product stimulates the activation of NLRP3 inflammasome and sparking the development of renal injury.

Yeh WJ, Yang HY, Pai MH, Wu CH, Chen JR
The Journal of nutritional biochemistry 2017 Jan;39:68-76

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Thrombomodulin Attenuates Inflammatory Damage Due to Liver Ischemia and Reperfusion Injury in Mice in Toll-Like Receptor 4-Dependent Manner.

Kadono K, Uchida Y, Hirao H, Miyauchi T, Watanabe T, Iida T, Ueda S, Kanazawa A, Mori A, Okajima H, Terajima H, Uemoto S
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2017 Jan;17(1):69-80

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Astragaloside IV attenuates cognitive impairments induced by transient cerebral ischemia and reperfusion in mice via anti-inflammatory mechanisms.

Li M, Li H, Fang F, Deng X, Ma S
Neuroscience letters 2017 Feb 3;639:114-119

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Stress hormone epinephrine (adrenaline) and norepinephrine (noradrenaline) effects on the anaerobic bacteria.

Boyanova L
Anaerobe 2017 Apr;44:13-19

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Transgenic expression of human APOL1 risk variants in podocytes induces kidney disease in mice.

Beckerman P, Bi-Karchin J, Park AS, Qiu C, Dummer PD, Soomro I, Boustany-Kari CM, Pullen SS, Miner JH, Hu CA, Rohacs T, Inoue K, Ishibe S, Saleem MA, Palmer MB, Cuervo AM, Kopp JB, Susztak K
Nature medicine 2017 Apr;23(4):429-438

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Carbon monoxide decreases interleukin-1β levels in the lung through the induction of pyrin.

Kim SK, Joe Y, Chen Y, Ryu J, Lee JH, Cho GJ, Ryter SW, Chung HT
Cellular & molecular immunology 2017 Apr;14(4):349-359

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Succinate/NLRP3 Inflammasome Induces Synovial Fibroblast Activation: Therapeutical Effects of Clematichinenoside AR on Arthritis.

Li Y, Zheng JY, Liu JQ, Yang J, Liu Y, Wang C, Ma XN, Liu BL, Xin GZ, Liu LF
Frontiers in immunology 2016;7:532

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Thioredoxin-Interacting Protein Mediates NLRP3 Inflammasome Activation Involved in the Susceptibility to Ischemic Acute Kidney Injury in Diabetes.

Xiao YD, Huang YY, Wang HX, Wu Y, Leng Y, Liu M, Sun Q, Xia ZY
Oxidative medicine and cellular longevity 2016;2016:2386068

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Ginsenoside Rb1 and compound K improve insulin signaling and inhibit ER stress-associated NLRP3 inflammasome activation in adipose tissue.

Chen W, Wang J, Luo Y, Wang T, Li X, Li A, Li J, Liu K, Liu B
Journal of ginseng research 2016 Oct;40(4):351-358

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The dominant-negative interplay between p53, p63 and p73: A family affair.

Billant O, Léon A, Le Guellec S, Friocourt G, Blondel M, Voisset C
Oncotarget 2016 Oct 25;7(43):69549-69564

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Inhibition of Mitochondrial Fission and NOX2 Expression Prevent NLRP3 Inflammasome Activation in the Endothelium: The Role of Corosolic Acid Action in the Amelioration of Endothelial Dysfunction.

Li Y, Zhou ZH, Chen MH, Yang J, Leng J, Cao GS, Xin GZ, Liu LF, Kou JP, Liu BL, Li P, Wen XD
Antioxidants & redox signaling 2016 Jun 1;24(16):893-908

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Hybrid inhibitor of peripheral cannabinoid-1 receptors and inducible nitric oxide synthase mitigates liver fibrosis.

Cinar R, Iyer MR, Liu Z, Cao Z, Jourdan T, Erdelyi K, Godlewski G, Szanda G, Liu J, Park JK, Mukhopadhyay B, Rosenberg AZ, Liow JS, Lorenz RG, Pacher P, Innis RB, Kunos G
JCI insight 2016 Jul 21;1(11)

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Itaconate Links Inhibition of Succinate Dehydrogenase with Macrophage Metabolic Remodeling and Regulation of Inflammation.

Lampropoulou V, Sergushichev A, Bambouskova M, Nair S, Vincent EE, Loginicheva E, Cervantes-Barragan L, Ma X, Huang SC, Griss T, Weinheimer CJ, Khader S, Randolph GJ, Pearce EJ, Jones RG, Diwan A, Diamond MS, Artyomov MN
Cell metabolism 2016 Jul 12;24(1):158-66

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Betaine prevented fructose-induced NAFLD by regulating LXRα/PPARα pathway and alleviating ER stress in rats.

Ge CX, Yu R, Xu MX, Li PQ, Fan CY, Li JM, Kong LD
European journal of pharmacology 2016 Jan 5;770:154-64

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Ilexgenin A inhibits endoplasmic reticulum stress and ameliorates endothelial dysfunction via suppression of TXNIP/NLRP3 inflammasome activation in an AMPK dependent manner.

Li Y, Yang J, Chen MH, Wang Q, Qin MJ, Zhang T, Chen XQ, Liu BL, Wen XD
Pharmacological research 2015 Sep;99:101-15

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Pharmacological activation of AMPK prevents Drp1-mediated mitochondrial fission and alleviates endoplasmic reticulum stress-associated endothelial dysfunction.

Li J, Wang Y, Wang Y, Wen X, Ma XN, Chen W, Huang F, Kou J, Qi LW, Liu B, Liu K
Journal of molecular and cellular cardiology 2015 Sep;86:62-74

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Induction of caspase-11 by aspartyl proteinases of Candida albicans and implication in promoting inflammatory response.

Gabrielli E, Pericolini E, Luciano E, Sabbatini S, Roselletti E, Perito S, Kasper L, Hube B, Vecchiarelli A
Infection and immunity 2015 May;83(5):1940-8

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Activation of the NLRP3 inflammasome in association with calcium oxalate crystal induced reactive oxygen species in kidneys.

Joshi S, Wang W, Peck AB, Khan SR
The Journal of urology 2015 May;193(5):1684-91

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Mangiferin inhibits endoplasmic reticulum stress-associated thioredoxin-interacting protein/NLRP3 inflammasome activation with regulation of AMPK in endothelial cells.

Song J, Li J, Hou F, Wang X, Liu B
Metabolism: clinical and experimental 2015 Mar;64(3):428-37

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Umbelliferone ameliorates cerebral ischemia-reperfusion injury via upregulating the PPAR gamma expression and suppressing TXNIP/NLRP3 inflammasome.

Wang X, Li R, Wang X, Fu Q, Ma S
Neuroscience letters 2015 Jul 23;600:182-7

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Curcumin attenuates glutamate neurotoxicity in the hippocampus by suppression of ER stress-associated TXNIP/NLRP3 inflammasome activation in a manner dependent on AMPK.

Li Y, Li J, Li S, Li Y, Wang X, Liu B, Fu Q, Ma S
Toxicology and applied pharmacology 2015 Jul 1;286(1):53-63

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Astragaloside IV and cycloastragenol are equally effective in inhibition of endoplasmic reticulum stress-associated TXNIP/NLRP3 inflammasome activation in the endothelium.

Zhao Y, Li Q, Zhao W, Li J, Sun Y, Liu K, Liu B, Zhang N
Journal of ethnopharmacology 2015 Jul 1;169:210-8

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Curcumin attenuates ethanol-induced hepatic steatosis through modulating Nrf2/FXR signaling in hepatocytes.

Lu C, Zhang F, Xu W, Wu X, Lian N, Jin H, Chen Q, Chen L, Shao J, Wu L, Lu Y, Zheng S
IUBMB life 2015 Aug;67(8):645-58

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Reactive oxygen species-induced TXNIP drives fructose-mediated hepatic inflammation and lipid accumulation through NLRP3 inflammasome activation.

Zhang X, Zhang JH, Chen XY, Hu QH, Wang MX, Jin R, Zhang QY, Wang W, Wang R, Kang LL, Li JS, Li M, Pan Y, Huang JJ, Kong LD
Antioxidants & redox signaling 2015 Apr 1;22(10):848-70

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Mizoribine ameliorates renal injury and hypertension along with the attenuation of renal caspase-1 expression in aldosterone-salt-treated rats.

Doi T, Doi S, Nakashima A, Ueno T, Yokoyama Y, Kohno N, Masaki T
PloS one 2014;9(4):e93513

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HIV and HCV activate the inflammasome in monocytes and macrophages via endosomal Toll-like receptors without induction of type 1 interferon.

Chattergoon MA, Latanich R, Quinn J, Winter ME, Buckheit RW 3rd, Blankson JN, Pardoll D, Cox AL
PLoS pathogens 2014 May;10(5):e1004082

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Betaine supplementation protects against high-fructose-induced renal injury in rats.

Fan CY, Wang MX, Ge CX, Wang X, Li JM, Kong LD
The Journal of nutritional biochemistry 2014 Mar;25(3):353-62

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Monocytes activate natural killer cells via inflammasome-induced interleukin 18 in response to hepatitis C virus replication.

Serti E, Werner JM, Chattergoon M, Cox AL, Lohmann V, Rehermann B
Gastroenterology 2014 Jul;147(1):209-220.e3

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Quercetin, luteolin and epigallocatechin gallate alleviate TXNIP and NLRP3-mediated inflammation and apoptosis with regulation of AMPK in endothelial cells.

Wu J, Xu X, Li Y, Kou J, Huang F, Liu B, Liu K
European journal of pharmacology 2014 Dec 15;745:59-68

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Secreted aspartic proteases of Candida albicans activate the NLRP3 inflammasome.

Pietrella D, Pandey N, Gabrielli E, Pericolini E, Perito S, Kasper L, Bistoni F, Cassone A, Hube B, Vecchiarelli A
European journal of immunology 2013 Mar;43(3):679-92

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Quercetin and allopurinol ameliorate kidney injury in STZ-treated rats with regulation of renal NLRP3 inflammasome activation and lipid accumulation.

Wang C, Pan Y, Zhang QY, Wang FM, Kong LD
PloS one 2012;7(6):e38285

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - Analysis of NLRP3 in cells (Huh-7 and SMMC-7721) exposed luteoloside using anti-NLRP3 antibody. Western blot image submitted by a verified customer review.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - Analysis of NALP3 using NALP3 antibody. Human testis lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse and 4) rat testis probed with NALP3 antibody at 5, 2 and 2 ug/mL respectively. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - Analysis of NALP3 using NALP3 antibody. Lane A) Human NALP3 transfected cell lysate, B) Mouse NALP3 transfected cell lysate, and C) HEK293 control lysate probed with NALP3 antibody at 3 ug/mL.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - Analysis in mouse cell lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - C-AR inhibited NLRP3 inflammasome activation in synovial tissue. NLRP3 protein expression in succinate-stimulated synovial fibroblasts. The results were derived from four independent experiments for immunohistochemistry staining and Western blot and expressed as the mean +/- SD. *p < 0.05 vs. the model; #p < 0.05 vs. the indicated treatment. Image collected and cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fimmu.2016.00532/full), licensed under a CC-BY licence.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - Regular aerobic exercise decreased myocardial inflammation in HFD rats. Representative Western blot analysis of NLRP3 and caspase-1. Image collected and cropped by CiteAb from the following publication (https://www.frontiersin.org/article/10.3389/fphys.2019.01286/full), licensed under a CC-BY licence.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP2-12446] - Analysis of human esophagus using NLRP3/NALP3 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining in the squamous epithelium was observed. Staining was performed by Histowiz.