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LearnMA5-16274
antibody from Invitrogen Antibodies
Targeting: NLRP3
AGTAVPRL, AII, AVP, C1orf7, CIAS1, CLR1.1, DFNA34, FCAS, FCU, MWS, NALP3, PYPAF1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
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- Product number
- MA5-16274 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-NLRP3 Monoclonal Antibody (25N10E9)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 25N10E9
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Apoptosis signal-regulating kinase-1 promotes inflammasome priming in macrophages.
Immanuel CN, Teng B, Dong B, Gordon EM, Kennedy JA, Luellen C, Schwingshackl A, Cormier SA, Fitzpatrick EA, Waters CM
American journal of physiology. Lung cellular and molecular physiology 2019 Mar 1;316(3):L418-L427
American journal of physiology. Lung cellular and molecular physiology 2019 Mar 1;316(3):L418-L427
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NLRP3 in mouse RAW lysate, recombinant mouse NLRP3 NOD domain protein immunogen. Samples were incubated in NLRP3 monoclonal antibody (Product # MA5-16274) using a dilution of 2 µg/mL followed by a Goat anti-mouse IgG HRP secondary antibody. Lane A and B: mouse RAW lysate; Lane C: recombinant mouse NLRP3 NOD domain protein immunogen; Lane D: primary antibody. PicoTect ECL substrate solution was used for this test.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NLRP3 in Raw 264 cell lysate. Samples were incubated in NLRP3 monoclonal antibody (Product # MA5-16274) using a dilution of 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NLRP3 in 0.5 mg/mL RAW lysate. Samples were incubated in NLRP3 monoclonal antibody (Product # MA5-16274). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
