PA1665

antibody from Boster Biological Technology

Targeting: NLRP3 AGTAVPRL, AII, AVP, C1orf7, CIAS1, CLR1.1, DFNA34, FCAS, FCU, MWS, NALP3, PYPAF1

Provider product page for PA1665

Western blot

Immunocytochemistry

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Flow cytometry

Antibody data

Antibody Data
Antigen structure
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Product number: PA1665 - Provider product page
Provider: Boster Biological Technology
Product name: Anti-CIAS1/NALP3/NLRP3 Antibody
Antibody type: Polyclonal
Description: Polyclonal antibody for NALP3/NLRP3 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. NALP3/NLRP3 information: Molecular Weight: 118173 MW; Subcellular Localization: Cytoplasm ; Tissue Specificity: Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Vial size: 100μg/vial
Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Handling: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

NLRP3 protein structure - PA1665 shown in red.

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Supportive validation

Submitted by: Boster Biological Technology (provider)
Main image
Experimental details: Western blot analysis of -CIAS1/NALP3 using anti- -CIAS1/NALP3 antibody (PA1665). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HEP-2 Cell Lysate Lane 2: A549 Cell Lysate Lane 3: U87 Cell Lysate Lane 4: CEM Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- -CIAS1/NALP3 antigen affinity purified polyclonal antibody (Catalog # PA1665) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for -CIAS1/NALP3 at approximately 118KD. The expected band size for -CIAS1/NALP3 is at 118KD.
Additional image