AF-515-NA
antibody from Novus Biologicals
Targeting: CXCL1
FSP, GRO1, GROa, MGSA, MGSA-a, NAP-3, SCYB1
Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunohistochemistry [2]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF-515-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal CXCL1/GRO alpha/KC/CINC-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects rat CXCL1/GRO alpha/KC/CINC-1 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse (rm) KC is observed and less than 10% cross-reactivity with recombinant human (rh) GRO beta , rhGRO gamma , rmMIP-2, recombinant rat (rr) CINC-2 alpha and rrCINC-3 is observed.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Involvement of cytokine-induced neutrophil chemoattractant in hypothalamic thermoregulation of luteinizing hormone-releasing hormone.
Rat pneumonia and soft-tissue infection models for the study of Acinetobacter baumannii biology.
Noguchi M, Yuzurihara M, Kase Y, Yasui T, Irahara M
Endocrinology 2008 Jun;149(6):2899-906
Endocrinology 2008 Jun;149(6):2899-906
Rat pneumonia and soft-tissue infection models for the study of Acinetobacter baumannii biology.
Russo TA, Beanan JM, Olson R, MacDonald U, Luke NR, Gill SR, Campagnari AA
Infection and immunity 2008 Aug;76(8):3577-86
Infection and immunity 2008 Aug;76(8):3577-86
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- CXCL1/GRO alpha/KC/CINC-1 in Rat Liver. CXCL1/GRO alpha/KC/CINC-1 was detected in perfusion fixed frozen sections of rat liver using Rat CXCL1/GRO alpha/KC/CINC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-515-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- CXCL1/GRO alpha/KC/CINC-1 in Rat Liver. CXCL1/GRO alpha/KC/CINC-1 was detected in perfusion fixed paraffin-embedded sections of rat liver using 15 µg/mL Rat CXCL1/GRO alpha/KC/CINC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-515-NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CXCL1/CINC-1 and Neutralization by Rat CXCL1/CINC-1 Antibody. Recombinant Rat CXCL1/CINC-1 (Catalog # 515-CN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL1/CINC-1 (4 ng/mL) is neutralized (green line) by increasing concentrations of Rat CXCL1/CINC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-515-NA). The ND50 is typically 0.15-0.75 µg/mL.