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antibody from Novus Biologicals
Targeting: CXCL1
FSP, GRO1, GROa, MGSA, MGSA-a, NAP-3, SCYB1
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [19]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF-453-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal CXCL1/GRO alpha/KC/CINC-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse CXCL1/GRO alpha/KC/CINC-1 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant rat CINC-1 is observed and less than 5% cross-reactivity with recombinant mouse (rm) MIP-2, recombinant human (rh) IL-8, rhGRO alpha , rhGRO beta and rmCXCL3 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references An IFNγ/CXCL2 regulatory pathway determines lesion localization during EAE.
Mycobacterium tuberculosis-triggered Hippo pathway orchestrates CXCL1/2 expression to modulate host immune responses.
Inducible Expression of CXCL1 within the Central Nervous System Amplifies Viral-Induced Demyelination.
Sphingolipid pathway regulates innate immune responses at the fetomaternal interface during pregnancy.
CXCR2-mediated tumor-associated neutrophil recruitment is regulated by IFN-β.
Altered regulation of Toll-like receptor responses impairs antibacterial immunity in the allergic lung.
CCL20, γδ T cells, and IL-22 in corneal epithelial healing.
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Detrimental effect of the proteasome inhibitor, bortezomib in bacterial superantigen- and lipopolysaccharide-induced systemic inflammation.
Intrinsic renal cell and leukocyte-derived TLR4 aggravate experimental anti-MPO glomerulonephritis.
Improved survival and reduced vascular permeability by eliminating or blocking 12/15-lipoxygenase in mouse models of acute lung injury (ALI).
Neuroprotection and remyelination after autoimmune demyelination in mice that inducibly overexpress CXCL1.
Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
Dachshund inhibits oncogene-induced breast cancer cellular migration and invasion through suppression of interleukin-8.
Respiratory syncytial virus induces insensitivity to beta-adrenergic agonists in mouse lung epithelium in vivo.
High pressure promotes monocyte adhesion to the vascular wall.
Interleukin-1 in combination with oncostatin M up-regulates multiple genes in chondrocytes: implications for cartilage destruction and repair.
High expression of ligands for chemokine receptor CXCR2 in alveolar epithelial neoplasia induced by oncogenic kras.
The chemokine KC, but not monocyte chemoattractant protein-1, triggers monocyte arrest on early atherosclerotic endothelium.
Stoolman JS, Duncker PC, Huber AK, Giles DA, Washnock-Schmid JM, Soulika AM, Segal BM
Journal of neuroinflammation 2018 Jul 16;15(1):208
Journal of neuroinflammation 2018 Jul 16;15(1):208
Mycobacterium tuberculosis-triggered Hippo pathway orchestrates CXCL1/2 expression to modulate host immune responses.
Boro M, Singh V, Balaji KN
Scientific reports 2016 Nov 24;6:37695
Scientific reports 2016 Nov 24;6:37695
Inducible Expression of CXCL1 within the Central Nervous System Amplifies Viral-Induced Demyelination.
Marro BS, Grist JJ, Lane TE
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 15;196(4):1855-64
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 15;196(4):1855-64
Sphingolipid pathway regulates innate immune responses at the fetomaternal interface during pregnancy.
Mizugishi K, Inoue T, Hatayama H, Bielawski J, Pierce JS, Sato Y, Takaori-Kondo A, Konishi I, Yamashita K
The Journal of biological chemistry 2015 Jan 23;290(4):2053-68
The Journal of biological chemistry 2015 Jan 23;290(4):2053-68
CXCR2-mediated tumor-associated neutrophil recruitment is regulated by IFN-β.
Jablonska J, Wu CF, Andzinski L, Leschner S, Weiss S
International journal of cancer 2014 Mar 15;134(6):1346-58
International journal of cancer 2014 Mar 15;134(6):1346-58
Altered regulation of Toll-like receptor responses impairs antibacterial immunity in the allergic lung.
Habibzay M, Saldana JI, Goulding J, Lloyd CM, Hussell T
Mucosal immunology 2012 Sep;5(5):524-34
Mucosal immunology 2012 Sep;5(5):524-34
CCL20, γδ T cells, and IL-22 in corneal epithelial healing.
Li Z, Burns AR, Miller SB, Smith CW
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Aug;25(8):2659-68
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Aug;25(8):2659-68
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
The Journal of clinical investigation 2010 Mar;120(3):907-23
The Journal of clinical investigation 2010 Mar;120(3):907-23
Detrimental effect of the proteasome inhibitor, bortezomib in bacterial superantigen- and lipopolysaccharide-induced systemic inflammation.
Tilahun AY, Theuer JE, Patel R, David CS, Rajagopalan G
Molecular therapy : the journal of the American Society of Gene Therapy 2010 Jun;18(6):1143-54
Molecular therapy : the journal of the American Society of Gene Therapy 2010 Jun;18(6):1143-54
Intrinsic renal cell and leukocyte-derived TLR4 aggravate experimental anti-MPO glomerulonephritis.
Summers SA, van der Veen BS, O'Sullivan KM, Gan PY, Ooi JD, Heeringa P, Satchell SC, Mathieson PW, Saleem MA, Visvanathan K, Holdsworth SR, Kitching AR
Kidney international 2010 Dec;78(12):1263-74
Kidney international 2010 Dec;78(12):1263-74
Improved survival and reduced vascular permeability by eliminating or blocking 12/15-lipoxygenase in mouse models of acute lung injury (ALI).
Zarbock A, Distasi MR, Smith E, Sanders JM, Kronke G, Harry BL, von Vietinghoff S, Buscher K, Nadler JL, Ley K
Journal of immunology (Baltimore, Md. : 1950) 2009 Oct 1;183(7):4715-22
Journal of immunology (Baltimore, Md. : 1950) 2009 Oct 1;183(7):4715-22
Neuroprotection and remyelination after autoimmune demyelination in mice that inducibly overexpress CXCL1.
Omari KM, Lutz SE, Santambrogio L, Lira SA, Raine CS
The American journal of pathology 2009 Jan;174(1):164-76
The American journal of pathology 2009 Jan;174(1):164-76
Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA Jr, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ
Proceedings of the National Academy of Sciences of the United States of America 2009 Apr 7;106(14):5954-9
Proceedings of the National Academy of Sciences of the United States of America 2009 Apr 7;106(14):5954-9
Dachshund inhibits oncogene-induced breast cancer cellular migration and invasion through suppression of interleukin-8.
Wu K, Katiyar S, Li A, Liu M, Ju X, Popov VM, Jiao X, Lisanti MP, Casola A, Pestell RG
Proceedings of the National Academy of Sciences of the United States of America 2008 May 13;105(19):6924-9
Proceedings of the National Academy of Sciences of the United States of America 2008 May 13;105(19):6924-9
Respiratory syncytial virus induces insensitivity to beta-adrenergic agonists in mouse lung epithelium in vivo.
Davis IC, Xu A, Gao Z, Hickman-Davis JM, Factor P, Sullender WM, Matalon S
American journal of physiology. Lung cellular and molecular physiology 2007 Aug;293(2):L281-9
American journal of physiology. Lung cellular and molecular physiology 2007 Aug;293(2):L281-9
High pressure promotes monocyte adhesion to the vascular wall.
Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D, Ninio E, van Haperen R, de Crom R, Tedgui A, Lehoux S
Circulation research 2007 Apr 27;100(8):1226-33
Circulation research 2007 Apr 27;100(8):1226-33
Interleukin-1 in combination with oncostatin M up-regulates multiple genes in chondrocytes: implications for cartilage destruction and repair.
Barksby HE, Hui W, Wappler I, Peters HH, Milner JM, Richards CD, Cawston TE, Rowan AD
Arthritis and rheumatism 2006 Feb;54(2):540-50
Arthritis and rheumatism 2006 Feb;54(2):540-50
High expression of ligands for chemokine receptor CXCR2 in alveolar epithelial neoplasia induced by oncogenic kras.
Wislez M, Fujimoto N, Izzo JG, Hanna AE, Cody DD, Langley RR, Tang H, Burdick MD, Sato M, Minna JD, Mao L, Wistuba I, Strieter RM, Kurie JM
Cancer research 2006 Apr 15;66(8):4198-207
Cancer research 2006 Apr 15;66(8):4198-207
The chemokine KC, but not monocyte chemoattractant protein-1, triggers monocyte arrest on early atherosclerotic endothelium.
Huo Y, Weber C, Forlow SB, Sperandio M, Thatte J, Mack M, Jung S, Littman DR, Ley K
The Journal of clinical investigation 2001 Nov;108(9):1307-14
The Journal of clinical investigation 2001 Nov;108(9):1307-14
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CXCL1/KC and Neutralization by Mouse CXCL1/KC Antibody. Recombinant Mouse CXCL1/KC (Catalog # 453-KC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/KC (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL1/KC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-453-NA). The ND50 is typically 0.3-1.5 µg/mL.
